| Description : |
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. |
| Source : |
human platelet |
| Species : |
Human |
| Form : |
The product is provided as a lyophilized white powder. |
| Bio-activity : |
The biological activity of non-muscle actin can be determined by its ability to efficiently polymerize into filaments in vitro and separate from unpolymerized components in a spin down assay. Stringent quality control ensures that >85% of the non-muscle actin can polymerized in this assay. |
| Molecular Mass : |
Non-muscle actin has an approximate molecular weight of 43 kDa. |
| Purity : |
Protein purity is determined by scanning densitometry of Coomassie Blue stained protein on a 12% polyacrylamide gel. Non-muscle actin was found to be >99% pure. |
| Applications : |
Identification and characterization of non-muscle actin binding proteins; In vitro actin polymerization studies; Antibody standard for Western blot analysis |
| Storage : |
Briefly centrifuge to collect the product at the bottom of the tube. The lyophilized protein is stable for 6 months when stored desiccated to <10% humidity at 4°C. |
| Reconstitution : |
The protein should be reconstituted to 10 mg/ml with 100 ul of distilled water, it will then be in the following buffer: 5 mM Tris-HCl pH 8.0, 0.2 mM CaCl2, 0.2 mM ATP, 5% (w/v) sucrose, and 1% (w/v) dextran. |
