Native Human ACTB

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Native Human ACTB

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Cat.No. : ACTB-325H
Product Overview : Non-muscle actin has been purified from human platelets. The isotype composition of non-muscle actin is 85% β-actin and 15% γ-actin.
Description : This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.
Source : human platelet
Species : Human
Form : The product is provided as a lyophilized white powder.
Bio-activity : The biological activity of non-muscle actin can be determined by its ability to efficiently polymerize into filaments in vitro and separate from unpolymerized components in a spin down assay. Stringent quality control ensures that >85% of the non-muscle actin can polymerized in this assay.
Molecular Mass : Non-muscle actin has an approximate molecular weight of 43 kDa.
Purity : Protein purity is determined by scanning densitometry of Coomassie Blue stained protein on a 12% polyacrylamide gel. Non-muscle actin was found to be >99% pure.
Applications : Identification and characterization of non-muscle actin binding proteins; In vitro actin polymerization studies; Antibody standard for Western blot analysis
Storage : Briefly centrifuge to collect the product at the bottom of the tube. The lyophilized protein is stable for 6 months when stored desiccated to <10% humidity at 4°C.
Reconstitution : The protein should be reconstituted to 10 mg/ml with 100 ul of distilled water, it will then be in the following buffer: 5 mM Tris-HCl pH 8.0, 0.2 mM CaCl2, 0.2 mM ATP, 5% (w/v) sucrose, and 1% (w/v) dextran.
Gene Name : ACTB actin, beta [ Homo sapiens (human) ]
Official Symbol : ACTB
Synonyms : ACTB; BRWS1; PS1TP5BP1; actin, beta; actin, cytoplasmic 1; beta cytoskeletal actin; PS1TP5-binding protein 1
Gene ID : 60
mRNA Refseq : NM_001101
Protein Refseq : NP_001092
MIM : 102630
UniProt ID : P60709
Chromosome Location : 7p22
Pathway : Adherens junction; Arrhythmogenic right ventricular cardiomyopathy; Bacterial invasion of epithelial cells
Function : Tat protein binding; kinesin binding; nitric-oxide synthase binding

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