Active Native Human ACTB

• Cat.No.: ACTB-325H
• Product Overview: Non-muscle actin has been purified from human platelets. The isotype composition of non-muscle actin is 85% β-actin and 15% γ-actin.

Specification

• Species: Human
• Source: Human Platelets
• Tag: Non
• Description: This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.
• Form: The product is provided as a lyophilized white powder.
• Bio-activity: The biological activity of non-muscle actin can be determined by its ability to efficiently polymerize into filaments in vitro and separate from unpolymerized components in a spin down assay. Stringent quality control ensures that >85% of the non-muscle actin can polymerized in this assay.
• Molecular Mass: Non-muscle actin has an approximate molecular weight of 43 kDa.
• Purity: Protein purity is determined by scanning densitometry of Coomassie Blue stained protein on a 12% polyacrylamide gel. Non-muscle actin was found to be >99% pure.
• Applications: Identification and characterization of non-muscle actin binding proteins; In vitro actin polymerization studies; Antibody standard for Western blot analysis
• Storage: Briefly centrifuge to collect the product at the bottom of the tube. The lyophilized protein is stable for 6 months when stored desiccated to <10% humidity at 4°C.
• Reconstitution: The protein should be reconstituted to 10 mg/ml with 100 ul of distilled water, it will then be in the following buffer: 5 mM Tris-HCl pH 8.0, 0.2 mM CaCl2, 0.2 mM ATP, 5% (w/v) sucrose, and 1% (w/v) dextran.

Gene Information

• Gene Name: ACTB actin, beta [ Homo sapiens (human) ]
• Official Symbol: ACTB
• Synonyms: ACTB; BRWS1; PS1TP5BP1; actin, beta; actin, cytoplasmic 1; beta cytoskeletal actin; PS1TP5-binding protein 1
• Gene ID: 60
• mRNA Refseq: NM_001101
• Protein Refseq: NP_001092
• MIM: 102630
• UniProt ID: P60709
• Chromosome Location: 7p22
• Pathway: Adherens junction; Arrhythmogenic right ventricular cardiomyopathy; Bacterial invasion of epithelial cells
• Function: Tat protein binding; kinesin binding; nitric-oxide synthase binding