Recombinant Human EPT1, GST-tagged

• Cat.No.: EPT1-102H
• Product Overview: Recombinant Human Ethanolaminephosphotransferase 1/EPT1 is produced by our E. coli expression system. The target protein is expressed with sequence (Met1-Pro50) of Human EPT1 fused with a GST tag at the N-terminus.

Specification

• Description: Ethanolaminephosphotransferase 1 (EPT1) is an enzyme that belongs to the CDP-Alcohol Phosphatidyltransferase Class-I Family. EPT1 is a Selenoprotein, which contains a Selenocysteine (Sec) residue at its active site. The Selenocysteine is encoded by the UGA codon that normally signals translation termination. The 3 UTR of Selenoprotein genes have a common stem-loop structure, the sec insertion sequence (SECIS), that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. EPT1 catalyzes Phosphatidylethanolamine biosynthesis from CDP-Ethanolamine. It plays a central role in the formation and maintenance of vesicular membranes. EPT1 is involved in the formation of Phosphatidylethanolamine via the Kennedy pathway.
• Source: E. coli
• Species: Human
• Tag: GST
• Form: Supplied as a 0.2 μM filtered solution of 20mM Tris-HCl, pH 8.0
• AA Sequence: MSPILGYWKIKGLVQPTRLLLEYLE EKYEEHLYERDEGDKWRNKKFELGL EFPNLPYYIDGDVKL TQSMAIIRYIADKHNMLGGCPKERA EISMLEGAVLDIRYGVSRIAYSKDF ETLKVDFLSKLPEML KMFEDRLCHKTYLNGDHVTHPDFML YDALDVVLYMDPMCLDAFPKLVCFK KRIEAIPQIDKYLKS SKYIAWPLQGWQATFGGGDHPPKSD LVPRGSPEFHMAGYEYVSPEQLAGF DKYKYSAVDTNPLSL YVMHPFWNTIVKVFPTWLAP
• Endotoxin: Less than 0.1 ng/μg (1 IEU/μg).
• Purity: Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
• Storage: Store at Please minimize freeze-thaw cycles.