Recombinant Human HSD17B4, His-tagged

• Cat.No.: HSD17B4-109H
• Product Overview: Recombinant Human Peroxisomal Multifunctional Enzyme Type 2/HSD17B4 is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (Gly2-Leu736) of Human HSD17B4 fused with a polyhistidine tag at the C-terminus.

Specification

• Description: Peroxisomal Multifunctional Enzyme Type 2 (MFE-2) belongs to the short-chain dehydrogenase/reductase (SDR) family. MFE-2 localizes to the peroxisome and contains one MaoC-like domain and one SCP2 domain. MFE-2 can be cleaved into (3R)-hydroxyacyl-CoA dehydrogenase and Enoyol-CoA hydratase 2 chains. MFE-2 acts as a bifunctional enzyme acting on the peroxisomal beta-oxidation pathway for fatty acids. It catalyzes the formation of 3-ketoacyl-CoA intermediates from both straight-chain and 2-methyl-branched-chain fatty acids. Defects in MFE-2 are a cause of D-bifunctional protein deficiency, which is a disorder of peroxisomal fatty acid β-oxidation.
• Source: HEK293
• Species: Human
• Tag: His
• AA Sequence: MGSPLRFDGRVVLVTGAGAGLGRAY ALAFAERGALVVVNDLGGDFKGVGK GSLAADKVVEEIRRR GGKAVANYDSVEEGEKVVKTALDAF GRIDVVVNNAGILRDRSFARISDED WDIIHRVHLRGSFQV TRAAWEHMKKQKYGRIIMTSSASGI YGNFGQANYSAAKLGLLGLANSLAI EGRKSNIHCNTIAPN AGSRMTQTVMPEDLVEALKPEYVAP LVLWLCHESCEENGGLFEVGAGWIG KLRWERTLGAIVRQK NHPMTPEAVKANWKKICDFENASKP QSIQESTGSIIEVLSKIDSEGGVSA NHTSRATSTATSGFA GAIGQKLPPFSYAYTELEAIMYALG VGASIKDPKDLKFIYEGSSDFSCLP TFGVIIGQKSMMGGG LAEIPGLSINFAKVLHGEQYLELYK PLPRAGKLKCEAVVADVLDKGSGVV IIMDVYSYSEKELIC HNQFSLFLVGSGGFGGKRTSDKVKV AVAIPNRPPDAVLTDTTSLNQAALY RLSGDWNPLHIDPNF ASLAGFDKPILHGLCTFGFSARRVL QQFADNDVSRFKAIKARFAKPVYPG QTLQTEMWKEGNRIH FQTKVQETGDIVISNAYVDLAPTSG TSAKTPSEGGKLQSTFVFEEIGRRL KDIGPEVVKKVNAVF EWHITKGGNIGAKWTIDLKSGSGKV YQGPAKGAADTTIILSDEDFMEVVL GKLDPQKAFFSGRLK ARGNIMLSQKLQMILKDYAKLVDHH HHHH
• Endotoxin: Less than 0.1 ng/μg (1 IEU/μg).
• Purity: Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.

Gene Information

• Gene Name: HSD17B4 hydroxysteroid (17-beta) dehydrogenase 4 [ Homo sapiens ]
• Official Symbol: HSD17B4
• Synonyms: HSD17B4; hydroxysteroid (17-beta) dehydrogenase 4; peroxisomal multifunctional enzyme type 2; 3 alpha; 7 alpha; 12 alpha trihydroxy 5 beta cholest 24 enoyl CoA hydratase; 17 beta HSD IV; 17 beta hydroxysteroid dehydrogenase 4; 17beta estradiol dehydrogenase type IV; beta hydroxyacyl dehydrogenase; beta keto reductase; D 3 hydroxyacyl CoA dehydratase; D bifunctional protein; peroxisomal; DBP; MFE 2; peroxisomal multifunctional protein 2; SDR8C1; short chain dehydrogenase/reductase family 8C; member 1; 17-beta-HSD 4; 17-beta-HSD IV; beta-keto-reductase; multifunctional protein 2; beta-hydroxyacyl dehydrogenase; D-3-hydroxyacyl-CoA dehydratase; D-bifunctional protein, peroxisomal; 17-beta-hydroxysteroid dehydrogenase 4; 17beta-estradiol dehydrogenase type IV; short chain dehydrogenase/reductase family 8C, member 1; 3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholest-24-enoyl-CoA hydratase; MFE-2; MPF-2
• Gene ID: 3295
• mRNA Refseq: NM_000414
• Protein Refseq: NP_000405
• MIM: 601860
• UniProt ID: P51659
• Chromosome Location: 5q2
• Pathway: Beta-oxidation of pristanoyl-CoA, organism-specific biosystem; Beta-oxidation of very long chain fatty acids, organism-specific biosystem; Bile acid and bile salt metabolism, organism-specific biosystem; Bile acid biosynthesis, cholesterol => cholate, organism-specific biosystem; Bile acid biosynthesis, cholesterol => cholate, conserved biosystem
• Function: 3-hydroxyacyl-CoA dehydrogenase activity; 3alpha,7alpha,12alpha-trihydroxy-5beta-cholest-24-enoyl-CoA hydratase activity; estradiol 17-beta-dehydrogenase activity; isomerase activity; long-chain-enoyl-CoA hydratase activity; lyase activity; nucleotide binding; oxidoreductase activity; sterol binding; sterol transporter activity