| Description : |
Semaphorin-4G is the least characterized of the seven known Class 4 transmembrane semaphorin glycoproteins. Class 4 semaphorins play multiple roles in cell attraction or repulsion, such as development of nerve pathways in the brain, promoting or inhibiting proliferation, in some cases organizing immune cell interactions. Semaphorin-4G can be expressed early in development in the central and peripheral nervous systems and in sensory ograns, such as cochlea, olfactory epithelium, vomeronasal organ and retina. In adults, Semaphorin-4G can be found in liver,kidney and brain. The human Semaphorin-4G precursor consists of a 17 amino acids signal sequence, a 658 amino acids extracellular domain, a 21 amino acids transmembrane domain, a 142 amino acids cytoplasmic domain with one Ser/Thr phosphorylation site. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
His |
| Form : |
Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4 |
| AA Sequence : |
VPGPSLRRPSRELDATPRMTIPYEE LSGTRHFKGQAQNYSTLLLEEASAR LLVGARGALFSLSAN DIGDGAHKEIHWEASPEMQSKCHQK GKNNQTECFNHVRFLQRLNSTHLYA CGTHAFQPLCAAIDA EAFTLPTSFEEGKEKCPYDPARGFT GLIIDGGLYTATRYEFRSIPDIRRS RHPHSLRTEETPMHW LNDAEFVFSVLVRESKASAVGDDDK VYYFFTERATEEGSGSFTQSRSSHR VARVARVCKGDLGGK KILQKKWTSFLKARLICHIPLYETL RGVCSLDAETSSRTHFYAAFTLSTQ WKTLEASAICRYDLA EIQAVFAGPYMEYQDGSRRWGRYEG GVPEPRPGSCITDSLRSQGYNSSQD LPSLVLDFVKLHPLM ARPVVPTRGRPLLLKRNIRYTHLTG TPVTTPAGPTYDLLFLGTADGWIHK AVVLGSGMHIIEETQ VFRESQSVENLVISLLQHSLYVGAP SGVIQLPLSSCSRYRSCYDCILARD PYCGWDPGTHACAAA TTIANRSQGSRTALIQDIERGNRGC ESSRDTGPPPPLKTRSVLRGDDVLL PCDQPSNLARALWLL NGSMGLSDGQGGYRVGVDGLLVTDA QPEHSGNYGCYAEENGLRTLLASYS LTVRPATPAPAPKAP ATPGAQLAPDVRLVDHHHHHH |
| Endotoxin : |
Less than 0.1 ng/μg (1 IEU/μg). |
| Purity : |
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. |
| Storage : |
Lyophilized protein should be stored at Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at |
| Reconstitution : |
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 μg/ml.Dissolve the lyophilized protein in 1X PBS.Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
