Recombinant Human TCF4, His-tagged

• Cat.No.: TCF4-5109H
• Product Overview: Recombinant human TCF4 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Specification

• Description: TCF4, also known as immunoglobulin transcription factor 2 and transcription factor 4, is a protein acting as a transcription factor. This protein recognizes an Ephrussi-box ("E-box") binding site ("CANNTG") - a motif first identified in immunoglobulin enhancers. It is expressed predominantly in pre-B-cells, although it is found in other tissues as well.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol 0.1M NaCl
• Molecular Mass: 56.6 kDa (530aa)
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMKFKQCR CSDTGLCCLD HEGKAEVYAP SASTADYNRD SPGYPSSKPA TSTFPSSFFM QDGHHSSDPW SSSSGMNQPG YAGMLGNSSH IPQSSSYCSL HPHERLSYPS HSSADINSSL PPMSTFHRSG TNHYSTSSCT PPANGTDSIM ANRGSGAAGS SQTGDALGKA LASIYSPDHT NNSFSSNPST PVGSPPSLSA GTAVWSRNGG QASSSPNYEG PLHSLQSRIE DRLERLDDAI HVLRNHAVGP STAMPGGHGD MHGIIGPSHN GAMGGLGSGY GTGLLSANRH SLMVGTHRED GVALRGSHSL LPNQVPVPQL PVQSATSPDL NPPQDPYRGM PPGLQGQSVS SGSSEIKSDD EGDENLQDTK SSEDKKLDDD KKDIKSITSN NDDEDLTPEQ KAEREKERRM ANNARERLRV RDINEAFKEL GRMVQLHLKS DKPQTKLLIL HQAVAVILSL EQQVRERNLN PKAACLKRRE EEKVSSEPPP LSLAGPHPGM GDASNHMGQM
• Purity: >90% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at +4centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20centigrade or -70centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: TCF4 transcription factor 4 [ Homo sapiens ]
• Official Symbol: TCF4
• Synonyms: TCF4; transcription factor 4; bHLHb19; E2 2; ITF2; SEF2 1B; SL3-3 enhancer factor 2; transcription factor 4, isoform D; transcription factor 4, isoform R; immunoglobulin transcription factor 2; class B basic helix-loop-helix protein 19; E2-2; PTHS; SEF2; ITF-2; SEF-2; TCF-4; SEF2-1; SEF2-1A; SEF2-1B; MGC149723; MGC149724
• Gene ID: 6925
• mRNA Refseq: NM_001083962
• Protein Refseq: NP_001077431
• MIM: 602272
• UniProt ID: P15884
• Chromosome Location: 18q21.1
• Pathway: CDO in myogenesis, organism-specific biosystem; Coregulation of Androgen receptor activity, organism-specific biosystem; Developmental Biology, organism-specific biosystem; Myogenesis, organism-specific biosystem; Regulation of Wnt-mediated beta catenin signaling and target gene transcription, organism-specific biosystem; Wnt Signaling Pathway NetPath, organism-specific biosystem
• Function: DNA binding; E-box binding; RNA polymerase II core promoter proximal region sequence-specific DNA binding; RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription; TFIIB-class binding transcription factor activity; TFIIB-class transcription factor binding; protein C-terminus binding; protein binding; protein heterodimerization activity; protein heterodimerization activity; sequence-specific DNA binding RNA polymerase recruiting transcription factor activity; sequence-specific DNA binding transcription factor activity