"Fgf1" Related Products

Recombinant Mouse Fgf1

Cat.No.: Fgf1-558M
Product Overview: Recombinant Mouse Fgf1 produced in E. coli is approximately 15.8 kDa, a single non-glycosylated polypeptide chain containing 140 amino acid residues.
Description: Murine aFGF, encoded by the FGF1 gene, is a member of the fibroblast growth factor (FGF) family. Fibroblast growth factor was found in pituitary extracts in 1973 and then tested in a bioassay that caused fibroblasts to proliferate. After further fractionating the extract using acidic and basic pH, two different forms have isolated that named "acidic fibroblast growth factor" (FGF-1) and "basic fibroblast growth factor" (FGF-2). Murine aFGF shares 52 % amino acid sequence identity with bFGF. Murine aFGF shares 96 % amino acid sequence identity with human aFGF, so it exhibits considerable species crossreactivity between murine and human aFGF. In mammalian FGF receptor family has 4 members, FGFR1, FGFR2, FGFR3, and FGFR4, and 1, 2, 3 have 2 sub-types "b", "c". aFGF can bind and activate all 7 different FGFRs. Affinity between aFGF and its receptors can be increased by heparin or heparan sulfate proteoglycan. aFGF plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. aFGF are also involved in a variety of biological processes, including embryonic development, morphogenesis, tissue repair, tumor growth and invasion.
Source: E. coli
Species: Mouse
Form: Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
Bio-activity: Fully biologically active when compared to standard. The ED 50 determined by a cell proliferation assay using murine balb/c 3T3 cells is less than 0.3 ng/ml, corresponding to a specific activity of >3.3 × 10^6 IU/mg in the presence of 10μg/ml of heparin.
Molecular Mass: 15.8 kDa
Endotoxin: Less than 1 EU/μg of rMuaFGF as determined by LAL method.
Purity: >96% by SDS-PAGE and HPLC analyses.
Usage: This material is for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
Storage: This lyophilized preparation is stable at 2-8 centigrade, but should be kept at -20 centigrade for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 centigrade. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 centigrade to -70 centigrade. Avoid repeated freeze/thaw cycles.
Reconstitution: We recommend that this vial be briefy centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at< -20 centigrade. further dilutions should be made in appropriate buffered solutions.>
Gene Name: Fgf1 fibroblast growth factor 1 [ Mus musculus ]
Official Symbol: Fgf1
Synonyms: FGF1; fibroblast growth factor 1; aFGF; HBGF-1; acidic fibroblast growth factor; heparin-binding growth factor 1; fibroblast growth factor 1 (acidic); Fam; Fgfa; Dffrx; Fgf-1;
Gene ID: 14164
mRNA Refseq: NM_010197
Protein Refseq: NP_034327
Pathway: Downstream signaling of activated FGFR, organism-specific biosystem; ESC Pluripotency Pathways, organism-specific biosystem; FGFR ligand binding and activation, organism-specific biosystem; FGFR1 ligand binding and activation, organism-specific biosystem; FGFR1b ligand binding and activation, organism-specific biosystem; FGFR1c ligand binding and activation, organism-specific biosystem; FGFR2 ligand binding and activation, organism-specific biosystem;
Function: S100 alpha binding; fibroblast growth factor receptor binding; growth factor activity; heparin binding; protein binding;

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