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Recombinant Mouse Cxcl3

Cat.No.: Cxcl3-610M
Product Overview: Recombinant Mouse Cxcl3 produced in E. coli is approximately 7.9 kDa, a single, non-glycosylated polypeptide chain containing 73 amino acids.
Description: Dendritic cell inflammatory protein-1 (DCIP-1)/CXCL3 is also known as MIP2β (macrophage inflammatory protein 2 beta), or DCIP1 (dendritic cell inflammatory protein-1) in mouse, CINC2 (cytokineinduced neutrophil attractant 2) in rat, and GROγ (growth regulated oncogene gamma) in humans. It is an 8 kDa proinflammatory member of the CXC subfamily of heparinbinding chemokines, also called alpha chemokines. Mature mouse CXCL3 shares 88% and 57% amino acid (aa) sequence identity with rat and human CXCL3, respectively.
Source: E. coli
Species: Mouse
Form: Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
Bio-activity: Fully biologically active when compared to standard. The ED 50 determined by a chemotaxis bioassay using human CXCR2 transfected human 293 cells is less than 100 ng/ml, corresponding to a specific activity of >1.0 × 10^4 IU/mg.
Molecular Mass: 7.9 kDa
AA Sequence: AVVASELRCQ CLNTLPRVDF ETIQSLTVTP PGPHCTQTEV IATLKDGQEV CLNPQGPRLQ IIIKKILKSG KSS
Endotoxin: Less than 1 EU/μg of rMuCXCL9 as determined by LAL method.
Purity: >97% by SDS-PAGE and HPLC analyses.
Usage: This material is for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
Storage: This lyophilized preparation is stable at 2-8 centigrade, but should be kept at -20 centigrade for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 centigrade. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 centigrade to -70 centigrade. Avoid repeated freeze/thaw cycles.
Reconstitution: We recommend that this vial be briefy centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 centigrade. Further dilutions should be made in appropriate buffered solutions.
Gene Name: Cxcl3 chemokine (C-X-C motif) ligand 3 [ Mus musculus ]
Official Symbol: Cxcl3
Synonyms: CXCL3; chemokine (C-X-C motif) ligand 3; C-X-C motif chemokine 3; dendritic cell inflammatory protein 1; Dcip1; Gm1960;
Gene ID: 330122
mRNA Refseq: NM_203320
Protein Refseq: NP_976065
Pathway: Chemokine receptors bind chemokines, organism-specific biosystem; Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; Cytokines and Inflammatory Response (BioCarta), organism-specific biosystem; G alpha (i) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem; GPCR ligand binding, organism-specific biosystem; Peptide ligand-binding receptors, organism-specific biosystem;
Function: chemokine activity; chemokine activity; cytokine activity; protein binding;

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