Recombinant Human BCKDK, GST-tagged

• Cat.No.: BCKDK-111H
• Product Overview: Recombinant human BCKDK (30-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Description: BCKDK or branched chain alpha-keto acid dehydrogenase kinase is an important regulator of the valine, leucine, and isoleucine catabolic pathways. BCKDK is found in the mitochondrion where it phosphorylates and inactivates BCKD. The branched-chain alpha-keto dehydrogenase complex (BCKDH) catalyzes the rate-limiting step in the oxidation of branched-chain amino acids and BCKDK inactivates the BCKDH complex by catalyzing serine phosphorylation of the E1-alpha subunit. BCKDH kinase catalyzes the serine phosphorylation of the E1- alpha subunit of BCKDH.
• Source: Sf9 insect cells using baculovirus
• Species: Human
• Tag: GST
• Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Molecular Mass: ~69 kDa
• Purity: >95% by densitometry
• Applications: Western Blot
• Storage: Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1 μg/μl

Gene Information

• Gene Name: BCKDK branched chain ketoacid dehydrogenase kinase [ Homo sapiens ]
• Official Symbol: BCKDK
• Synonyms: BCKDK; branched chain ketoacid dehydrogenase kinase; [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial; BCKDHKIN; BCKD-kinase; branched chain alpha-ketoacid dehydrogenase kinase; branched-chain alpha-ketoacid dehydrogenase kinase
• Gene ID: 10295
• mRNA Refseq: NM_001122957
• Protein Refseq: NP_001116429
• UniProt ID: O14874
• Chromosome Location: 16p11.2
• Function: ATP binding; ATP binding; [3-methyl-2-oxobutanoate dehydrogenase (acetyl-transferring)] kinase activity; kinase activity; kinase activity; nucleotide binding; protein binding; protein serine/threonine kinase activity; two-component sensor activity