Recombinant Full Length Human BCKDK Protein, C-Flag-tagged

• Cat.No.: BCKDK-538HFL
• Product Overview: Recombinant Full Length Human BCKDK Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The branched-chain alpha-ketoacid dehydrogenase complex (BCKD) is an important regulator of the valine, leucine, and isoleucine catabolic pathways. The protein encoded by this gene is found in the mitochondrion, where it phosphorylates and inactivates BCKD. Several transcript variants encoding different isoforms have been found for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 46.2 kDa
• AA Sequence: MILASVLRSGPGGGLPLRPLLGPAL ALRARSTSATDTHHVEMARERSKTV TSFYNQSAIDAAAEKPSVRL TPTMMLYAGRSQDGSHLLKSARYLQ QELPVRIAHRIKGFRCLPFIIGCNP TILHVHELYIRAFQKLTDFP PIKDQADEAQYCQLVRQLLDDHKDV VTLLAEGLRESRKHIEDEKLVRYFL DKTLTSRLGIRMLATHHLAL HEDKPDFVGIICTRLSPKKIIEKWV DFARRLCEHKYGNAPRVRINGHVAA RFPFIPMPLDYILPELLKNA MRATMESHLDTPYNVPDVVITIANN DVDLIIRISDRGGGIAHKDLDRVMD YHFTTAEASTQDPRISPLFG
  HLDMHSGAQSGPMHGFGFGLPTSRA YAEYLGGSLQLQSLQGIGTDVYLRL RHIDGREESFRITRTRPLEQKLISE EDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Protein Kinase

Gene Information

• Gene Name: BCKDK branched chain keto acid dehydrogenase kinase [ Homo sapiens (human) ]
• Official Symbol: BCKDK
• Synonyms: BDK; BCKDKD
• Gene ID: 10295
• mRNA Refseq: NM_005881.4
• Protein Refseq: NP_005872.2
• MIM: 614901
• UniProt ID: O14874