Recombinant Human PIKFYVE, GST-tagged

• Cat.No.: PIKFYVE-120H
• Product Overview: Recombinant human PIP5K3 (1493-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Description: PIP5K3 (also known as phosphatidylinositol-3-phosphate 5-kinase type III or PIPKIII) encodes an enzyme that phosphorylates the D-5 position in PtdIns and phosphatidylinositol-3-phosphate (PtdIns3P) to make PtdIns5P and PtdIns(3,5)biphosphate. PIP5K3 belongs to a large family of lipid kinases that alter the phosphorylation status of intracellular phosphatidylinositol but also have protein kinase activity. PIP5K3 regulate cytoskeletal functions, membrane trafficking, and receptor signaling by recruiting protein complexes to cell- and endosomal-membranes. The inhibition of PIP5K3 renders the late endosome/lysosome compartment refractory to fusion with autophagosomes and multivesicular bodies.
• Source: Sf9 insect cells using baculovirus
• Species: Human
• Tag: GST
• Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Molecular Mass: ~ 93 kDa
• Purity: >75% by densitometry
• Applications: Western Blot
• Storage: Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1 μg/μl

Gene Information

• Gene Name: PIKFYVE phosphoinositide kinase, FYVE finger containing [ Homo sapiens ]
• Official Symbol: PIKFYVE
• Synonyms: PIKFYVE; phosphoinositide kinase, FYVE finger containing; phosphatidylinositol 3 phosphate/phosphatidylinositol 5 kinase, type III , PIP5K3; 1-phosphatidylinositol 3-phosphate 5-kinase; KIAA0981; MGC40423; p235; PIKfyve; PIP5K; PIPkin-III; type III PIP kinase; phosphatidylinositol 3-phosphate 5-kinase; 1-phosphatidylinositol-3-phosphate 5-kinase; phosphatidylinositol 3-phosphate 5-kinase type III; phosphatidylinositol-3-phosphate/phosphatidylinositol 5-kinase, type III; CFD; FAB1; PIP5K3; FLJ37746
• Gene ID: 200576
• mRNA Refseq: NM_001178000
• Protein Refseq: NP_001171471
• MIM: 609414
• UniProt ID: Q9Y2I7
• Chromosome Location: 2q34
• Pathway: 3-phosphoinositide biosynthesis, organism-specific biosystem; 3-phosphoinositide biosynthesis, conserved biosystem; Inositol phosphate metabolism, organism-specific biosystem; Inositol phosphate metabolism, conserved biosystem; Phagosome, organism-specific biosystem; Phagosome, conserved biosystem; Phosphatidylinositol signaling system, organism-specific biosystem
• Function: 1-phosphatidylinositol-3-phosphate 5-kinase activity; 1-phosphatidylinositol-4-phosphate 5-kinase activity; ATP binding; metal ion binding; nucleotide binding; protein binding; transferase activity; zinc ion binding