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Active Recombinant Human DNMT3A, GST-tagged

Cat.No. : DNMT3A-164H
Product Overview : Recombinant full-length human DNMT3A was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
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Description : DNMT3A: DNA (cytosine-5-)-methyltransferase 3 alpha plays important role in genomic imprinting and X chromosome inactivation and is essential for mammalian development. DNMT3A appears to function as a de novo methyltrabsferase, since it can methylate unmethylated and hemimethylated DNA with equal efficiencies. DNMT3A is required for genome wide de novo methylation and is essential for mammalian development. DNMT3A is critical for maintaining active chromatin states of genes that are essential for development.
Source : Sf9 insect cells using baculovirus
Species : Human
Tag : GST
Form : Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Bio-activity : The specific activity of DNMT3A was determined to be 250 pmol /min/mg
Molecular Mass : ~162 kDa
Purity : >70% by densitometry
Applications : Methyltransferase Assay
Storage : Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Concentration : 0.1 μg/μl
Gene Name : DNMT3A DNA (cytosine-5-)-methyltransferase 3 alpha [ Homo sapiens ]
Official Symbol : DNMT3A
Synonyms : DNMT3A; DNA (cytosine-5-)-methyltransferase 3 alpha; DNA (cytosine-5)-methyltransferase 3A; DNA MTase HsaIIIA; DNA cytosine methyltransferase 3A2; DNMT3A2; M.HsaIIIA;
Gene ID : 1788
mRNA Refseq : NM_022552
Protein Refseq : NP_072046
MIM : 602769
UniProt ID : Q9Y6K1
Chromosome Location : 2p23
Pathway : Cysteine and methionine metabolism, organism-specific biosystem; Cysteine and methionine metabolism, conserved biosystem; Metabolic pathways, organism-specific biosystem; Methionine degradation, organism-specific biosystem; Methionine degradation, conserved biosystem; One Carbon Metabolism, organism-specific biosystem; Validated targets of C-MYC transcriptional repression, organism-specific biosystem;
Function : DNA (cytosine-5-)-methyltransferase activity; DNA (cytosine-5-)-methyltransferase activity, acting on CpG substrates; DNA binding; chromatin binding; metal ion binding; methyltransferase activity; protein binding; transferase activity; unmethylated CpG binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (5)

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Can DNMT3A protein be used as a biomarker for cancer diagnosis? 02/23/2022

DNMT3A mutations can serve as biomarkers for certain cancers, aiding in the diagnosis and classification of hematologic malignancies.

How does DNMT3A impact normal cellular function? 04/15/2021

DNMT3A is essential for normal DNA methylation patterns during development and differentiation, influencing gene expression and cellular function.

Can DNMT3A-targeted therapies have off-target effects? 06/18/2020

Like many targeted therapies, DNMT3A-targeted treatments may have off-target effects, and their specificity is an area of active research.

Are there diagnostic tests for detecting DNMT3A mutations? 05/28/2020

Yes, molecular diagnostic tests, such as DNA sequencing, can identify DNMT3A mutations in cancer cells.

How does DNMT3A contribute to epigenetic changes in cancer cells? 03/03/2020

DNMT3A mutations lead to abnormal DNA methylation patterns, contributing to altered gene expression and promoting cancer development.

Customer Reviews (3)

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11/17/2022

    Its exceptional characteristics, coupled with the manufacturer's commitment to customer satisfaction, ensure that I can pursue my scientific investigations with confidence and success.

    09/07/2022

      Its consistent performance and reproducibility have been praised by scientists who have employed it in their studies, with many reporting outstanding results and a high level of satisfaction.

      08/20/2017

        This level of support becomes invaluable, allowing researchers to focus on their scientific objectives while receiving expert guidance alongside.

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