Cat.No.:	UBA3-254H
Product Overview:	Recombinant full-length human UBA3 (UBE1C) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Description:	UBA3 (also known as UBE1C) or ubiquitin-like modifier activating enzyme 3 is a member of the E1 ubiquitin-activating enzyme family. UBA3 associates with AppBp1, an amyloid beta precursor protein binding protein, to form a heterodimer, and then the enzyme complex activates NEDD8, a ubiquitin-like protein, which regulates cell division, signaling and embryogenesis. The mechanism of NEDD8 activation by APPBP1-UBA3 shows a high degree of conservation with ubiquitin activation by UBA1. The NEDD8-activating enzyme, or NAE, composed of NAE1 and UBA3 subunits, is an essential component for the treatment of cancer disease.
Source:	Sf9 insect cells using baculovirus
Species:	Human
Tag:	GST
Form:	Recombinant protein stored in 50mM Tis-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Molecular Mass:	~73 kDa
Purity:	>95% by densitometry
Applications:	Western Blot
Storage:	Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Concentration:	0.1 μg/μl

Gene Name:	UBA3 ubiquitin-like modifier activating enzyme 3 [ Homo sapiens ]
Official Symbol:	UBA3
Synonyms:	UBA3; ubiquitin-like modifier activating enzyme 3; UBE1C, ubiquitin activating enzyme E1C (homologous to yeast UBA3) , ubiquitin activating enzyme E1C (UBA3 homolog, yeast); NEDD8-activating enzyme E1 catalytic subunit; hUba3; ubiquitin activating enzyme E1 homolog (yeast); NEDD8-activating enzyme E1C; Nedd8-activating enzyme hUba3; ubiquitin-activating enzyme 3; ubiquitin-like modifier-activating enzyme 3; UBA3, ubiquitin-activating enzyme E1 homolog; ubiquitin-activating enzyme E1C (UBA3 homolog, yeast); ubiquitin-activating enzyme E1C (homologous to yeast UBA3); UBE1C; hUBA3; MGC22384; DKFZp566J164;
Gene ID:	9039
mRNA Refseq:	NM_003968
Protein Refseq:	NP_003959
MIM:	603172
UniProt ID:	Q8TBC4
Chromosome Location:	3p24.3-p13
Pathway:	Adaptive Immune System, organism-specific biosystem; Antigen processing: Ubiquitination & Proteasome degradation, organism-specific biosystem; Class I MHC mediated antigen processing & presentation, organism-specific biosystem; Coregulation of Androgen receptor activity, organism-specific biosystem; Immune System, organism-specific biosystem;
Function:	ATP binding; NEDD8 activating enzyme activity; acid-amino acid ligase activity; ligase activity; nucleotide binding; protein binding; protein heterodimerization activity;

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