Recombinant Mouse Kdm4b, GST-tagged

• Cat.No.: Kdm4b-260M
• Product Overview: Recombinant mouse KDM4B (1-725) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Description: KDM4B or lysine (K)-specific demethylase 4B, which is also known as JMJD2B, contains a JmjN domain, a JmjC domain, a JD2H domain, 2 TUDOR domains, and a bipartite nuclear localization signal that overlaps the C-terminal part of the second TUDOR domain. KDM4B plays an essential role in human carcinogenesis through positive regulation of cyclin-dependent kinase 6. KDM4B functions as a co-factor of estrogen receptor in breast cancer proliferation and mammary gland development. KDM4B is regulated by both ER? and HIF-1?, drives breast cancer cell proliferation in normoxia and hypoxia, and epigenetically regulates the expression of cell cycle genes such as CCND1, CCNA1, and WEE1.
• Source: Sf9 insect cells using baculovirus
• Species: Mouse
• Tag: GST
• Form: Recombinant protein stored in 50mM Tis-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Molecular Mass: ~125 kDa
• Purity: >75% by densitometry
• Applications: Western Blot
• Storage: Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1 μg/μl

Gene Information

• Gene Name: Kdm4b lysine (K)-specific demethylase 4B [ Mus musculus ]
• Official Symbol: Kdm4b
• Synonyms: KDM4B; lysine (K)-specific demethylase 4B; lysine-specific demethylase 4B; jumonji domain containing 2B; jumonji domain-containing protein 2B; jmjC domain-containing histone demethylation protein 3B; Jmjd2b; mKIAA0876; MGC6349; 4732474L06Rik
• Gene ID: 193796
• mRNA Refseq: NM_172132
• Protein Refseq: NP_742144
• Function: metal ion binding; oxidoreductase activity; oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen; zinc ion binding