Active Recombinant Mouse Il4, Fc-tagged

• Cat.No.: Il4-316M
• Product Overview: The extracellular domain of mouse IL-4 (aa 21-140) is fused to the N-terminus of the Fc region of a mutant mouse IgG2a.

Specification

• Description: Interleukin-4 (IL-4) is a cytokine produced by type 2 helper T cells, the Th2 cells. These cells tends to make a specific set of lymphokines including IL-4, IL-5, IL-6, IL-10, IL-13, IL-3 and GM-CSF and fail to produce IL-2, IFN-γ, and lymphotoxin (TNF-beta). In addition, mast cells can produce IL-4. IL-4 exerts numerous effects on various hematopoietic cell types. On B cells, IL-4 promotes immunoglobulin class switching to IgE and IgG1 isotypes and upregulates MHC class II and CD23 expression. IL-4 promotes survival, growth, and differentiation of both T and B lymphocytes, mast cells and endothelial cells. In addition, IL-4 inhibits the production of TNF, IL-1, and IL-6 by macrophages.
• Source: CHO Cells
• Species: Mouse
• Tag: Fc
• Form: Lyophilized from 0.2μm-filtered solution in PBS.
• Bio-activity: Shows the biological function of the IL-4 moiety and exerts a prolonged circulating half-life caused by the modified Fc domain.
• Purity: ≥98% (SDS-PAGE)
• Stability: Stable for at least 1 year after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.
• Storage: Short Term Storage: +4°C; Long Term Storage: -20°C. Avoid freeze/thaw cycles.

Gene Information

• Gene Name: Il4 interleukin 4 [ Mus musculus ]
• Official Symbol: Il4
• Synonyms: IL4; interleukin 4; interleukin-4; IGG1 induction factor; B-cell growth factor 1; B-cell stimulatory factor 1; lymphocyte stimulatory factor 1; B-cell IgG differentiation factor; Il-4; BSF-1
• Gene ID: 16189
• mRNA Refseq: NM_021283
• Protein Refseq: NP_067258
• Pathway: Allograft rejection, organism-specific biosystem; Allograft rejection, conserved biosystem; Asthma, organism-specific biosystem; Asthma, conserved biosystem; Autoimmune thyroid disease, organism-specific biosystem; Autoimmune thyroid disease, conserved biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem
• Function: cytokine activity; cytokine receptor binding; growth factor activity; interleukin-4 receptor binding

Reviews

10/17/2021

By testing immunogenicity in animal models, detect whether an immune response is induced.

11/09/2020

The structure of the recombinant protein was analyzed by nuclear magnetic resonance (NMR), X-ray crystallography or electron microscopy, and the results showed that the protein was stable.

05/06/2020

This protein has good stability, and the test results of heat resistance, acid and alkali resistance and protease degradation resistance are good.