Recombinant Human TNF Protein, FLAG-tagged

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Recombinant Human TNF Protein, FLAG-tagged

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Cat.No. : TNF-408H
Product Overview : Recombinant protein of human TNF with tracellular domain of human TNF-α (aa 85-233) is fused at the N-terminus to mouse ACRP30headless (aa 18-111) and a FLAG®-tag.
Description : This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.
Source : HEK293T
Species : Human
Tag : FLAG
Form : Lyophilized
Bio-activity : Activates human and mouse TNF-R1 and TNF-R2. Induces cell death at a concentration range of 0.01-10 ng/ml (ED50= 0.05ng/ml or 2 x 10E7 Units/mg; WEHI 164 cells).
Molecular Mass : ~34kDa (SDS-PAGE)
Endotoxin : <0.02 EU/μg purified protein (LAL test; Lonza).
Purity : ≥95% (SDS-PAGE)
Stability : Stable for at least 6 months after receipt when stored at -20 centigrade. Working aliquots are stable for up to 3 months when stored at -20 centigrade.
Storage : Short Term Storage: 4 centigrade.
Long Term Storage: -20 centigrade.
Concentration : 0.1 mg/mL after reconstitution.
Storage Buffer : PBS
Reconstitution : Reconstitute with 100 μL sterile water.
Handling : After reconstitution, prepare aliquots and store at -20 centigrade. Avoid freeze/thaw cycles. PBS containing at least 0.1% BSA should be used for further dilutions.
Gene Name : TNF tumor necrosis factor [ Homo sapiens (human) ]
Official Symbol : TNF
Synonyms : TNF; tumor necrosis factor; DIF; TNFA; TNFSF2; TNLG1F; TNF-alpha; tumor necrosis factor; APC1 protein; TNF, macrophage-derived; TNF, monocyte-derived; TNF-a; cachectin; tumor necrosis factor ligand 1F; tumor necrosis factor ligand superfamily member 2; tumor necrosis factor-alpha
Gene ID : 7124
mRNA Refseq : NM_000594
Protein Refseq : NP_000585
MIM : 191160
UniProt ID : P01375

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