Recombinant Human CD200 protein, Fc-tagged

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Recombinant Human CD200 protein, Fc-tagged

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Cat.No. : CD200-165H
Product Overview : Recombinant Human CD200 protein (Gln31-Gly232) was fused to human IgG1 Fc tag at C-terminus and expressed in human 293 cells (HEK293).
Description : CD200 is also known as OX-2 membrane glycoprotein (OX-2), is a type-1 membrane glycoprotein, which contains two immunoglobulin domains (1 Ig-like C2-type (immunoglobulin-like) domain and Ig-like V-type (immunoglobulin-like) domain), and thus belongs to the immunoglobulin superfamily. CD200/OX-2 is widely expressed in multiple cell types. CD200 interacts with a structurally related receptor (CD200R) expressed mainly on myeloid cells and is involved in regulation of macrophage and mast cell function. OX-2/CD200 and CD200R associate via their respective N-terminal Ig-like domains. CD200 also plays an important role in prevention of graft rejection, autoimmune diseases and spontaneous abortion.
Source : HEK293
Species : Human
Tag : human IgG1 Fc
Predicted N Terminal : Gln 31
Form : Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5, 10% trehalose.
Molecular Mass : The protein has a calculated MW of 48.6 kDa. The protein migrates as 50-68 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein length : Gln31-Gly232
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE of CD200-165H :

Human CD200, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA of CD200-165H :

Immobilized Human CD200, Fc Tag at 2 μg/mL (100 μL/well) can bind Biotinylated Human CD200 R1, Fc,Avitag with a linear range of 0.5-7.8 ng/mL.

Gene Name : CD200
Official Symbol : CD200
Synonyms : MRC; MOX1; MOX2; OX-2
Gene ID : 4345
mRNA Refseq : NM_005944.7
Protein Refseq : NP_005935.4
MIM : 155970
UniProt ID : P41217
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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