||Recombinant Human Pim-3 fused with 6His tag at Nterminal, was expressed in Sf21 insect cells.
||The protein encoded by this gene belongs to the Ser/Thr protein kinase family, and PIM subfamily. This gene is overexpressed in hematological and epithelial tumors and is associated with MYC coexpression. It plays a role in the regulation of signal transduction cascades, contributing to both cell proliferation and survival, and provides a selective advantage in tumorigenesis.
||Sf21 insect cells
||1.05mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
||5210U/mg, where one unit of Pim-3 activity is defined as 1nmol phosphate incorporated into 300µM (RSRHSSYPAGT) per minute at 30°C with a final ATP concentration of 100µM.
||Stable for 6 months at -70°C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap.