Recombinant Human KRT5, His-tagged

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Recombinant Human KRT5, His-tagged

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Cat.No. : KRT5-147H
Product Overview : KRT5, 1-590aa, Human, His tag, E.coli
Description : KRT5 is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. Recombinant human KRT5 protein, fused to His-tag at N-terminus, was expressed in E.coli
Source : E.coli
Species : Human
Tag : His-tag
Form : Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.4M Urea
Molecular Mass : 64.8 kDa (613 aa)
Protein length : 1-590aa
AA Sequence : MGSSHHHHHH SSGLVPRGSH MGSMSRQSSV SFRSGGSRSF STASAITPSV SRTSFTSVSR SGGGGGGGFG RVSLAGACGV GGYGSRSLYN LGGSKRISIS TSGGSFRNRF GAGAGGGYGF GGGAGSGFGF GGGAGGGFGL GGGAGFGGGF GGPGFPVCPP GGIQEVTVNQ SLLTPLNLQI DPSIQRVRTE EREQIKTLNN KFASFIDKVR FLEQQNKVLD TKWTLLQEQG TKTVRQNLEP LFEQYINNLR RQLDSIVGER GRLDSELRNM QDLVEDFKNK YEDEINKRTT AENEFVMLKK DVDAAYMNKV ELEAKVDALM DEINFMKMFF DAELSQMQTH VSDTSVVLSM DNNRNLDLDS IIAEVKAQYE EIANRSRTEA ESWYQTKYEE LQQTAGRHGD DLRNTKHEIS EMNRMIQRLR AEIDNVKKQC ANLQNAIADA EQRGELALKD ARNKLAELEE ALQKAKQDMA RLLREYQELM NTKLALDVEI ATYRKLLEGE ECRLSGEGVG PVNISVVTSS VSSGYGSGSG YGGGLGGGLG GGLGGGLAGG GSGSYYSSSS GGVGLSGGLS VGGSGFSASS GRGLGVGFGS GGGSSSSVKF VSTTSSSRKS FKS
Purity : >85 % by SDS - PAGE
Storage : Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Concentration : 1 mg/ml (determined by Bradford assay)
Gene Name : KRT5 keratin 5 [ Homo sapiens ]
Official Symbol : KRT5
Synonyms : KRT5; keratin 5; EBS2, epidermolysis bullosa simplex 2 Dowling Meara/Kobner/Weber Cockayne types , keratin 5 (epidermolysis bullosa simplex, Dowling Meara/Kobner/Weber Cockayne types); keratin, type II cytoskeletal 5; KRT5A; CK-5; cytokeratin-5; 58 kda cytokeratin; type-II keratin Kb5; epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types; keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types); K5; CK5; DDD; EBS2;
Gene ID : 3852
mRNA Refseq : NM_000424
Protein Refseq : NP_000415
MIM : 148040
UniProt ID : P13647
Chromosome Location : 12q13.13
Pathway : Glucocorticoid receptor regulatory network, organism-specific biosystem;
Function : protein binding; structural constituent of cytoskeleton;
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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