Recombinant Human ZWINT, His-tagged

• Cat.No.: ZWINT-153H
• Product Overview: ZWINT, 1-230aa, Human, His tag, E.coli

Specification

• Description: ZW10 interactor isoform b, also known as ZWINT, is clearly involved in kinetochore function. Localized to the cytoplasm during interphase and to kinetochores from late prophase to anaphase, ZWINT interacts with ZW10 (Zeste White 10) and functions to regulate the association between ZW10 and kinetochores. Defects in the gene encoding ZWINT are associated with the pathogenesis of Roberts’s syndrome, an autosomal recessive disorder characterized by growth retardation due to premature chromosome separation. Recombinant human ZWINT protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Source: E.coli
• Species: Human
• Tag: His-tag
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT
• Molecular Mass: 27.9 kDa (253aa) confirmed by MALDI-TOF (Molecular size on SDS-PAGE will appear higher)
• Protein length: 1-230aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMEAAETE AEAAALEVLA EVAGILEPVG LQEEAELPAK ILVEFVVDSQ KKDKLLCSQL QVADFLQNIL AQEDTAKGLD PLASEDTSRQ KAIAAKEQWK ELKATYREHV EAIKIGLTKA LTQMEEAQRK RTQLREAFEQ LQAKKQMAME KRRAVQNQWQ LQQEKHLQHL AEVSAEGKLL FPEAEAEAEN LPDDKPQQPT RPQEQSTGDT MGRDPGVSFK AVGLQPAGDV NLP
• Purity: >85% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: ZWINT ZW10 interactor [ Homo sapiens ]
• Official Symbol: ZWINT
• Synonyms: ZWINT; ZW10 interactor; KNTC2AP; zwint-1; ZW10-interacting protein 1; human ZW10 interacting protein-1; ZWINT1; HZwint-1; MGC117174
• Gene ID: 11130
• mRNA Refseq: NM_001005413
• Protein Refseq: NP_001005413
• MIM: 609177
• UniProt ID: O95229
• Chromosome Location: 10q21-q22
• Pathway: Cell Cycle, organism-specific biosystem; Cell Cycle, Mitotic, organism-specific biosystem; DNA Replication, organism-specific biosystem; M Phase, organism-specific biosystem; Mitotic M-M/G1 phases, organism-specific biosystem; Mitotic Prometaphase, organism-specific biosystem
• Function: protein N-terminus binding; protein binding