Recombinant Human HERC5, His-tagged

• Cat.No.: HERC5-178H
• Product Overview: HERC5, 681-1024aa, Human, His tag, E.coli

Specification

• Description: HERC5 is a member of the HERC family of ubiquitin ligases. It is a protein with a HECT domain and five RCC1 repeats. The protein localizes to the cytoplasm and perinuclear region and functions as an interferon-induced E3 protein ligase that mediates ISGylation of protein targets. HERC5 gene lies in a cluster of HERC family genes on chromosome 4. It has been shown to exhibit antiviral activity towards HIV-1, influenza A virus and human papillomavirus. Recombinant human HERC5 protein, fused to His-tag at N-terminus, was expressed in E.coli.
• Source: E.coli
• Species: Human
• Tag: His-tag
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.4M Urea, 10% glycerol
• Molecular Mass: 43 kDa (367aa)
• Protein length: 681-1024aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSFDLTVRR NHLIEDVLNQ LSQFENEDLR KELWVSFSGE IGYDLGGVKK EFFYCLFAEM IQPEYGMFMY PEGASCMWFP VKPKFEKKRY FFFGVLCGLS LFNCNVANLP FPLALFKKLL DQMPSLEDLK ELSPDLGKNL QTLLDDEGDN FEEVFYIHFN VHWDRNDTNL IPNGSSITVN QTNKRDYVSK YINYIFNDSV KAVYEEFRRG FYKMCDEDII KLFHPEELKD VIVGNTDYDW KTFEKNARYE PGYNSSHPTI VMFWKAFHKL TLEEKKKFLV FLTGTDRLQM KDLNNMKITF CCPESWNERD PIRALTCFSV LFLPKYSTME TVEEALQEAI NNNRGFG
• Purity: >90% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: HERC5 HECT and RLD domain containing E3 ubiquitin protein ligase 5 [ Homo sapiens ]
• Official Symbol: HERC5
• Synonyms: HERC5; HECT and RLD domain containing E3 ubiquitin protein ligase 5; hect domain and RLD 5; E3 ISG15--protein ligase HERC5; CEB1; cyclin-E-binding protein 1; probable E3 ubiquitin-protein ligase HERC5; HECT domain and RCC1-like domain-containing protein 5; CEBP1
• Gene ID: 51191
• mRNA Refseq: NM_016323
• Protein Refseq: NP_057407
• MIM: 608242
• UniProt ID: Q9UII4
• Chromosome Location: 4q22.1-q23
• Pathway: Antiviral mechanism by IFN-stimulated genes, organism-specific biosystem; Cytokine Signaling in Immune system, organism-specific biosystem; ISG15 antiviral mechanism, organism-specific biosystem; Immune System, organism-specific biosystem; Innate Immune System, organism-specific biosystem; Interferon Signaling, organism-specific biosystem; Negative regulators of RIG-I/MDA5 signaling, organism-specific biosystem
• Function: ISG15 ligase activity; acid-amino acid ligase activity; ligase activity; protein binding; ubiquitin-protein ligase activity