Species : |
Human |
Source : |
E.coli |
Tag : |
His |
Protein Length : |
1-311aa |
Description : |
Serine/threonine-protein kinase pim-2, also known as PIM2, is a serine/threonine protein kinase in the CAMK family. PIM2 is expressed in many tissues, with high levels in spleen, thymus, testis, small intestine, and colon. It is implicated in tumor phenotypes and may be involved in the formation and preservation of Long-Term Potentiation (LTP), a profuse, activity-dependent enhancement of synaptic efficacy that is implicated in long-term memory. Recombinant human PIM2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
Form : |
Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol |
Molecular Mass : |
36.6 kDa (334aa) confirmed by MALDI-TOF |
AA Sequence : |
MGSSHHHHHH SSGLVPRGSH MGSMLTKPLQ GPPAPPGTPT PPPGGKDREA FEAEYRLGPL LGKGGFGTVF AGHRLTDRLQ VAIKVIPRNR VLGWSPLSDS VTCPLEVALL WKVGAGGGHP GVIRLLDWFE TQEGFMLVLE RPLPAQDLFD YITEKGPLGE GPSRCFFGQV VAAIQHCHSR GVVHRDIKDE NILIDLRRGC AKLIDFGSGA LLHDEPYTDF DGTRVYSPPE WISRHQYHAL PATVWSLGIL LYDMVCGDIP FERDQEILEA ELHFPAHVSP DCCALIRRCL APKPSSRPSL EEILLDPWMQ TPAEDVPLNP SKGGPAPLAW SLLP |
Purity : |
>85% by SDS - PAGE |
Storage : |
Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles. |
Concentration : |
0.25 mg/ml (determined by Bradford assay) |