Recombinant Human GNRH1, His-tagged

• Cat.No.: GNRH1-219H
• Product Overview: GNRH1, 24-92aa, Human, His tag, E.coli

Specification

• Description: GNRH1 is secreted and then cleaved to form the 10 aa luteinizing hormone-releasing hormone (LHRH, also known as gonadoliberin-1), and prolactin release-inhibiting factor (also known as GnRH-associated peptide 1). GNRH1 stimulates the release of luteinizing and follicle stimulating hormones, which are important for reproduction. The protein stimulates the secretion of gonadotropins; it stimulates the secretion of both luteinizing and follicle-stimulating hormones. Recombinant human GNRH1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Source: E.coli
• Species: Human
• Tag: His-tag
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT
• Molecular Mass: 10.3 kDa (92aa) confirmed by MALDI-TOF
• Protein length: 24-92aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSQHWSYGL RPGGKRDAEN LIDSFQEIVK EVGQLAETQR FECTTHQPRS PLRDLKGALE SLIEEETGQK KI
• Purity: >90% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: GNRH1 gonadotropin-releasing hormone 1 (luteinizing-releasing hormone) [ Homo sapiens ]
• Official Symbol: GNRH1
• Synonyms: GNRH1; gonadotropin-releasing hormone 1 (luteinizing-releasing hormone); GNRH, gonadotropin releasing hormone 1 (leutinizing releasing hormone) , GRH, LHRH; progonadoliberin-1; luliberin I; progonadoliberin I; GnRH-associated peptide 1; prolactin release-inhibiting factor; gonadotropin-releasing hormone 1 (leutinizing-releasing hormone); GRH; GNRH; LHRH; LNRH
• Gene ID: 2796
• mRNA Refseq: NM_000825
• Protein Refseq: NP_000816
• MIM: 152760
• UniProt ID: P01148
• Chromosome Location: 8p21-p11.2
• Pathway: Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; G alpha (q) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem; GPCR ligand binding, organism-specific biosystem; GnRH signaling pathway, organism-specific biosystem; GnRH signaling pathway, conserved biosystem; Hormone ligand-binding receptors, organism-specific biosystem
• Function: gonadotropin hormone-releasing hormone activity; hormone activity