Active Recombinant Human SPAM1 protein, His-tagged
Cat.No. : | SPAM1-738H |
Product Overview : | Recombinant Human SPAM1 protein(Leu 36 - Tyr 482), fused with His tag, was expressed in HEK293. |
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Source : | HEK293 |
Species : | Human |
Tag : | His |
Form : | Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM NaCl, pH7.5, 10% trehalose. |
Bio-activity : | The activity of Human PH20, His Tag is measured by its ability to hydrolyze HA in turbidimetric assay (45 minute assay). The specific activity is >60,000 U/mg. (Unit Definition: One unit of Hyaluronidase activity will cause a change in A600 of 0.330 per minute at pH5.35 at 37 °C in a 2.0 mL reaction mixture). |
Molecular Mass : | This protein carries a polyhistidine tag at the C-terminus.The protein has a calculated MW of 53.0 kDa. The protein migrates as 60-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Protein Length : | Leu 36 - Tyr 482 |
Endotoxin : | Less than 1.0 EU per μg by the LAL method. |
Purity : | >95% as determined by SDS-PAGE. |
Storage : | For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
Gene Name : | SPAM1 sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding) [ Homo sapiens ] |
Official Symbol : | SPAM1 |
Synonyms : | SPAM1; sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding); hyaluronidase PH-20; HYAL5; PH 20; SPAG15; hyal-PH20; sperm surface protein PH-20; hyaluronoglucosaminidase PH-20; HYA1; PH20; HYAL1; HYAL3; PH-20; MGC26532; |
Gene ID : | 6677 |
mRNA Refseq : | NM_001174044 |
Protein Refseq : | NP_001167515 |
MIM : | 600930 |
UniProt ID : | P38567 |
Products Types
◆ Recombinant Protein | ||
SPAM1-5341R | Recombinant Rat SPAM1 Protein, His (Fc)-Avi-tagged | +Inquiry |
SPAM1-8607M | Recombinant Mouse SPAM1 Protein, His (Fc)-Avi-tagged | +Inquiry |
SPAM1-15H | Recombinant Human SPAM1 Protein, C-Myc/DDK-tagged | +Inquiry |
Spam1-2092M | Recombinant Mouse Spam1 Protein, His-tagged | +Inquiry |
SPAM1-01H | Recombinant Human SPAM1 Protein, C-Myc/DDK-tagged | +Inquiry |
◆ Lysates | ||
SPAM1-1546HCL | Recombinant Human SPAM1 293 Cell Lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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