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| Cat.No.: | CD274-256H |
| Product Overview: | Human PDL1 /CD274 Protein, Biotinylated (Biotinylated PDL1 / CD274) Phe 19 - Arg 238, was labeled with biotin based on rhPDL1 Protein, which was produced in human 293 cells (HEK293). |
| Description: | Programmed cell death 1 ligand 1 (PD-L1) also known as cluster of differentiation (CD274) or B7 homolog 1 (B7-H1), is a member of the growing B7 family of immune molecules and is involved in the regulation of cellular and humoral immune responses. B7-H1 is a cell surface immunoglobulin superfamily with two Ig-like domains within the extracellular region and a short cytoplasmic domain. PD-L1 is highly expressed in the heart, skeletal muscle, placenta and lung and weakly expressed in the thymus, spleen, kidney and liver. PD-L1 is expressed on activated T-cells, B-cells, dendritic cells, keratinocytes and monocytes. PD-L1 is up-regulated on T- and B-cells, dendritic cells, keratinocytes and monocytes after LPS and IFNG activation and up-regulated in B-cells activated by surface Ig cross-linking. PD-L1 involve in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. |
| Source: | HEK293 |
| Species: | Human |
| Tag: | His |
| Form: | Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Trehalose are added as protectants before lyophilization. |
| Molecular Mass: | Biotinylated PDL1 / CD274, fused with 6×His tag at the C-terminus, has a calculated MW of 26 kDa. The predicted N-terminus is Phe 19. DTT-reduced Protein migrates as 35-42 kDa due to glycosylation. |
| Endotoxin: | Less than 1.0 EU per μg of the Biotinylated PDL1 / CD274 by the LAL method. |
| Purity: | >95% as determined by reduced SDS-PAGE. |
| Storage: | Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C). |
| Gene Name: | CD274 CD274 molecule [ Homo sapiens ] |
| Official Symbol: | CD274 |
| Synonyms: | CD274; CD274 molecule; CD274 antigen , PDCD1LG1, programmed cell death 1 ligand 1; programmed cell death 1 ligand 1; B7 homolog 1; B7 H; B7 H1; B7H1; PD L1; PDL1; CD274 antigen; PDCD1 ligand 1; programmed death ligand 1; B7-H; PD-L1; PDCD1L1; PDCD1LG1; MGC142294; MGC142296; |
| Gene ID: | 29126 |
| mRNA Refseq: | NM_014143 |
| Protein Refseq: | NP_054862 |
| MIM: | 605402 |
| UniProt ID: | Q9NZQ7 |
| Chromosome Location: | 9p24.1 |
| Pathway: | Adaptive Immune System, organism-specific biosystem; Cell adhesion molecules (CAMs), organism-specific biosystem; Cell adhesion molecules (CAMs), conserved biosystem; Costimulation by the CD28 family, organism-specific biosystem; Immune System, organism-specific biosystem; PD-1 signaling, organism-specific biosystem; |
| Function: | protein binding; protein tyrosine phosphatase activity; receptor activity; |
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