|Product Overview :
||Recombinant Human CAII /CA2 MS Standard Protein, C13 and N15-labeled (CAII / CA2, Heavy Labeled) Met 1 - Lys 260 (Accession # NP_000058.1) was produced in human 293 cells (HEK293).
||Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes. CAs form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons (or vice versa), a reversible reaction that occurs rather slowly in the absence of a catalyst. One of the functions of the enzyme in animals is to interconvert carbon dioxide and bicarbonate to maintain acid-base balance in blood and other tissues, and to help transport carbon dioxide out of tissues. The active site of most carbonic anhydrases contains a zinc ion. They are, therefore, classified as metalloenzymes. There are at least five distinct CA families (α, β, γ, δ and ε). These families have no significant amino acid sequence similarity and in most cases are thought to be an example of convergent evolution. The α-CAs are found in humans. Carbonic anhydrase II (CA2) also known as Carbonate dehydratase II, Carbonic anhydrase C, is one of fourteen forms of human α carbonic anhydrases. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. Renal carbonic anhydrase allows the reabsorption of sodium ions in the proximal tubule. Carbonic anhydrase II has been shown to interact with Band 3 and Sodium-hydrogen antiporter 1.[2-5]
||Lyophilized from 0.22 μm filtered solution in 20 mM Tris, pH 8.0, with 150 mM NaCl, 1 mM DTT.. Normally Mannitol or Trehalose are added as protectants before lyophilization.
|Molecular Mass :
||CAII / CA2, Heavy Labeled, fused with 6×His tag at the C-terminus, has a calculated MW of 30 kDa. The predicted N-terminus is Met 1 . DTT-reduced Protein migrates as 30 kDa.
||Less than 1.0 EU per μg of the CAII / CA2, Heavy Labeled by the LAL method.
||>95% as determined by SDS-PAGE.
||Avoid repeated freeze-thaw cycles.No activity loss was observed after storage at:In lyophilized state for 1 year (4oC-8oC); After reconstitution under sterile conditions for 1 month (4oC-8oC) or 3 months (-20oC to -70oC).