||Recombinant Human GPA33 MS Standard Protein, C13 and N15-labeled (GPA33, Heavy Labeled) Ile 22 - Val 235 (Accession # AAH74830) was produced in human 293 cells (HEK293).
||Glycoprotein A33 (GPA33) also known as Cell surface A33 antigen, is a single-pass type I membrane protein which is expressed in normal gastrointestinal epithelium and in 95% of colon cancers. GPA33 The predicted mature protein has a 213-amino acid extracellular region, a single transmembrane domain, and a 62-amino acid intracellular tail. The sequence of the extracellular region contains 1 Ig-like C2-type (immunoglobulin-like) domain and 1 Ig-like V-type (immunoglobulin-like) domain characteristic of the CD2 subgroup of the immunoglobulin (Ig) superfamily, which contains. GPA33 may play a role in cell-cell recognition and signaling.
||Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.
||GPA33, Heavy Labeled, fused with 6×His tag at the C-terminus, has a calculated MW of 24.5 kDa. The predicted N-terminus is Ile 22. DTT-reduced Protein migrates as 35-40 kDa due to glycosylation.
||Less than 1.0 EU per μg of the GPA33, Heavy Labeled by the LAL method.
||>96% as determined by SDS-PAGE.
||Avoid repeated freeze-thaw cycles.No activity loss was observed after storage at:In lyophilized state for 1 year (4oC-8oC); After reconstitution under sterile conditions for 1 month (4oC-8oC) or 3 months (-20oC to -70oC).