Recombinant Human PIWIL2 protein, His-tagged

• Cat.No.: PIWIL2-1737H
• Product Overview: Recombinant Human PIWIL2 protein(662-973 aa), fused with His tag, was expressed in E.coli.

Specification

• Source: E.coli
• Species: Human
• Tag: His
• Form: The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH7.4). 5 % trehalose and 5 % mannitol are added as protectant before lyophilization. The elution buffer contain 300mM imidazole.
• Protein Length: 662-973 aa
• AA Sequence: AEGKIQMVVCIIMGPRDDLYGAIKK LCCVQSPVPSQVVNVRTIGQPTRLR SVAQKILLQINCKLGGELWGVDIPL KQLMVIGMDVYHDPSRGMRSVVGFV ASINLTLTKWYSRVVFQMPHQEIVD SLKLCLVGSLKKFYEVNHCLPEKIV VYRDGVSDGQLKTVANYEIPQLQKC FEAFENYQPKMVVFVVQKKISTNLY LAAPQNFVTPTPGTVVDHTITSCEW VDFYLLAHHVRQGCGIPTHYVCVLN TANLSPDHMQRLTFKLCHMYWNWPG TIRVPAPCKYAHKLAFLSGHILHHE PAIQLCENLFFL
• Purity: 85%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Short-term storage: Store at 2-8°C for (1-2 weeks).
  Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
• Reconstitution: Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
  Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage.

Gene Information

• Gene Name: PIWIL2 piwi-like 2 (Drosophila) [ Homo sapiens ]
• Official Symbol: PIWIL2
• Synonyms: PIWIL2; piwi-like 2 (Drosophila); piwi-like protein 2; cancer/testis antigen 80; CT80; FLJ10351; HILI; Hiwi like; Mili; Miwi like; piwil2-like protein; mili; PIWIL1L; MGC133049
• Gene ID: 55124
• mRNA Refseq: NM_001135721
• Protein Refseq: NP_001129193
• MIM: 610312
• UniProt ID: Q8TC59