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Recombinant Mouse resistin like gamma, His-tagged

Cat.No. : Retnlg-122M
Product Overview : Recombinant Mouse RELM-gamma is a His-Tagged Fusion Protein having a molecular weight of 11 kDa containing 86 amino acid residues of the RELM-gamma Mouse and 16 additional amino acid residues–HisTag.
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  • Gene Information
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Cat. No. : Retnlg-122M
Description : RELM-gamma is a novel member of the resistin-like molecule/found in inflammatory zone (RELM/FIZZ) family in mice and rats. Microarray and real-time RT-PCR experiments revealed a repression of RELMgamma mRNA in nasal respiratory epithelium of cigarette smoke-exposed versus untreated rats. The analysis of the physiological tissue-specific expression revealed highest expression in hematopoietic tissues, suggesting a cytokine-like role for RELM-gamma. RELM-gamma-mRNA is detectable in bone marrow, spleen, and lung as well as in peripheral blood granulocytes. Promyelocytic HL60 cells transfected with a RELM-gamma expression plasmid have an increased proliferation rate compared to mock-transfected cells and display an altered response to retinoic acid-induced granulocytic differentiation. Taken together, these data provide the first experimental evidence that RELM-gamma is a secreted molecule with a restricted expression pattern that may play a role in promyelocytic differentiation.
Source : Escherichia Coli.
Amino acid sequence : MRGSHHHHHH GMASHMTLES IVEKKVKELL ANRDDCPSTV TKTFSCTSIT ASGRLASCPS GMTVTGCACG YGCGSWDIRD GNTCHCQCST MDWATARCCQ LA.
Specificity : The amino acid sequence of the recombinant Mouse RELM is 100% homologous to the amino acid sequence of the RELM-Mouse.
Purification Method : Two-step procedure using size exclusion chromatography before and after refolding.
Purity : >95% (SDS-PAGE analyzed).
Formulation : Sterile filtered and lyophilized from 0.5 mg/ml in 0.05M Acetate buffer pH-4.
Applications : Western blotting, ELISA
Solubility : Add 0.2 ml of 0.1 M Acetate buffer pH-4 and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10 μg/ml. In higher concentrations the solubility of this antigen is limited.
Stability : Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C. The lyophilized protein remains stable until the expiry date when stored at -20°C.
Gene Name : Retnlg resistin like gamma [ Mus musculus ]
Synonyms : Retnlg; resistin like gamma; Xcp1; Fizz3; Relmg; resistin-like gamma; RELMgamma; resistin-like molecule gamma; ADSF; HXCP1; MGC126603; MGC126609; RETN1; RSTN; resistin
Gene ID : 245195
mRNA Refseq : NM_181596
Protein Refseq : NP_853627
UniProt ID : Q7TM98
Chromosome Location : 16 B5; 16 32.5 cM
Function : molecular_function

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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