|Product Overview:||Recombinant Human MSLN(Glu 296 - Gly 580) fused with His tag at C-terminal was expressed in HEK293.|
|Description:||Mesothelin (MSLN) is also known as CAK1 antigen, Pre-pro-megakaryocyte-potentiating factor, which belongs to the mesothelin family. Mesothelin / MSLN can be proteolytically cleaved into the following two chains by a furin-like convertase: Megakaryocyte-potentiating factor (MPF) and the cleaved form of mesothelin. Both MPF and the cleaved form of mesothelin are N-glycosylated. Mesothelin / MSLN can interacts with MUC16. The membrane-anchored forms of MSLN may play a role in cellular adhesion. MPF potentiates megakaryocyte colony formation in vitro.|
|Predicted N Terminal:||Glu 296|
|Form:||Lyophilized from 0.22µm filtered solution in PBS (pH 7.4) with Trehalose as protectant.|
|Molecular Mass:||The protein has a calculated MW of 33 kDa. The protein migrates as 38-42 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.|
|Protein length:||Glu 296 - Gly 580|
|Endotoxin:||Less than 1.0 EU per μg by the LAL method.|
|Purity:||>95% as determined by SDS-PAGE.|
|Notes:||The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.|
|Storage:||For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower. Please avoid repeated freeze-thaw cycles.
No activity loss is observed after storage at:
4-8 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
|Reconstitution:||Reconstitute at 100 μg/mL in sterile deionized water. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the COA.|
|Gene Name:||MSLN mesothelin [ Homo sapiens ]|
|Synonyms:||MSLN; mesothelin; CAK1; MPF; CAK1 antigen; megakaryocyte potentiating factor; soluble MPF mesothelin related protein; pre-pro-megakaryocyte-potentiating factor; SMRP;|