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Active Recombinant Human MSLN Protein (Glu 296 - Gly 580), Fc-tagged, FITC-Labeled

Cat.No. : MSLN-316H
Product Overview : Recombinant Human MSLN Protein, Fc-tagged, FITC-Labeled is expressed from human HEK293 cells. It contains AA Glu 296 - Gly 580 (Accession # AAH09272.1). It is the FITC labeled form of Human Mesothelin (296-580), Fc Tag. Predicted N-terminus: Glu 296. This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 58.6 kDa. The protein migrates as 60-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. Conjugate (FITC): Excitation source: 488 nm spectral line, argon-ion laser; Excitation Wavelength: 488 nm; Emission Wavelength: 535 nm. The FITC to protein molar ratio is 1-2.5. The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular seive treatment during purification process.
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Source : HEK293
Species : Human
Tag : Fc
Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Bio-activity : Immobilized Human Mesothelin (296-580), Fc Tag, premium grade (Cat. No. MSN-H5253) at 0.5 μg/mL (100 μL/well) can bind Anti-Human MSLN Mab with a linear range of 0.1-3 ng/mL.
Molecular Mass : The protein has a calculated MW of 58.6 kDa. The protein migrates as 66-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein Length : Glu 296 - Gly 580
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 centigrade to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.
Gene Name : MSLN mesothelin [ Homo sapiens ]
Official Symbol : MSLN
Synonyms : MSLN; mesothelin; CAK1; MPF; CAK1 antigen; megakaryocyte potentiating factor; soluble MPF mesothelin related protein; pre-pro-megakaryocyte-potentiating factor; SMRP;
Gene ID : 10232
mRNA Refseq : NM_001177355
Protein Refseq : NP_001170826
MIM : 601051
UniProt ID : Q13421

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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What is the primary role of MSLN in normal tissues? 12/07/2022

MSLN is normally expressed in mesothelial cells, playing a role in cell adhesion.

How is MSLN expression altered in cancerous cells? 03/19/2022

In cancer, MSLN expression is significantly upregulated.

In which types of cancer is MSLN overexpressed? 03/28/2021

MSLN is overexpressed in mesothelioma, ovarian, and pancreatic cancers.

Are there any therapeutic strategies targeting MSLN in cancer treatment? 02/25/2020

Therapies targeting MSLN include antibody-drug conjugates and CAR-T cell therapies.

How can MSLN levels be detected and measured in clinical settings? 10/07/2019

MSLN levels can be assessed through immunohistochemistry and serum biomarker tests.

What is the prognostic significance of MSLN overexpression in cancer patients? 11/23/2018

MSLN overexpression is often associated with poor prognosis in cancer patients.

How does MSLN contribute to cancer progression and metastasis? 11/07/2018

MSLN promotes tumor cell proliferation, invasion, and resistance to apoptosis.

Customer Reviews (3)

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Reviews
05/22/2022

    Very satisfied, great product.

    03/06/2022

      Product is high-quality.

      09/30/2021

        Excellent for experiments, reliable.

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