Creative BioMart to Present at
                        BIO-Europe Spring Creative BioMart to Present at AACR Annual Meeting|Apr. 5-10, 2024|Booth #2953

Active Recombinant Human INHBA, Animal Free

Cat.No. : INHBA-131H
Product Overview : Recombinant human Activin A is a 27.4 kDa disulphide-linked homodimers of two beta-A chains, each containing 116 amino residues. Human recombinant protein expressed in Nicotiana benthamiana. Recombinant human Activin A contains a 6-His-tag at the N-terminal end, is produced by transient expression in non-transgenic plants and is purified by sequential chromatography (FPLC). This product contains no animal–derived components or impurities. Animal free product.
  • Specification
  • Gene Information
  • Related Products
Description : Activins are homodimers or heterodimers of the various beta subunit isoforms, belonging to the TGFbeta family. Mature Activin A has two 116 amino acids residues betaA subunits (betaA-betaA). Activin exhibits a wide range of biological activities, including mesoderm induction, neural cell differentiation, bone remodelling, haematopoiesis, and reproductive physiology. Activins plays a key role in the production and regulation of hormones such as FSH, LH, GnRH and ACTH. Cells known to express Activin A include fibroblasts, endothelial cells, hepatocytes, vascular smooth muscle cells, macrophages, keratinocytes, osteoclasts, bone marrow monocytes, prostatic epithelium, neurons, chondrocytes, osteoblasts, Leydig cells, Sertoli cells, and ovarian granulosa cells.As with other members of the super-family, Activins interact with two types of cell surface trans-membrane receptors (Types I and II) which have intrinsic serine/threonine kinase activities in their cytoplasmic domains, Activin type 1 receptors, ACVR1, ACVR1B, ACVR1C and Activin type 2 receptors, ACVR2A, ACVR2B. The biological activity of Activin A can be neutralized by inhibins and by the diffusible TGF-B antagonist, Follistatin.
Source : Nicotiana benthamiana
Species : Human
Form : Lyophilized from a Tris HCl 0.05M buffer at pH 7.4.
Bio-activity : The biological activity of Activin A is measured by its ability to inhibit mouse plasmacytoma cell line (MPC-11) cells proliferation ([3H]thymidine incorporation). ED50 ≤ 5 ng/ml.
Molecular Mass : Recombinant human Activin A is a 27.4 kDa disulphide-linked homodimers of two beta-A chains, each containing 116 amino residues.
AA Sequence : HHHHHHGLECDGKVNICCKKQFFVS FKDIGWNDWIIAPSGYHANYCEGEC PSHIAGTSGSSLSFHSTVINHYRMR GHSPFANLKSCCVPTKLRPMSMLYY DDGQNIIKKDIQNMIVEECGCS
Endotoxin : < 0.04 eu ug protein (lal>
Purity : >97% by SDS-PAGE gel
Applications : Cell culture, Western blot
Storage : This lyophilized preparation is stable at 2-8o C for short term, long storage it should be kept at -20oC. Reconstituted protein should be stored in working aliquots at –20°C and it is recommended to add a carrier protein (0.1% HSA or BSA). Repeated freezing and thawing is not recommended.
Reconstitution : Lyophilized protein should be reconstituted in water to a concentration of 50 ng/ul. Due to the protein nature, dimmers and multimers may be observed. Upon reconstitution, It can be stored in working aliquots at –20°C for future use. Optimal reconstitution please follow batch Quality Control sheet instructions.
Gene Name : INHBA inhibin, beta A [ Homo sapiens ]
Official Symbol : INHBA
Synonyms : INHBA; inhibin, beta A; inhibin, beta A (activin A, activin AB alpha polypeptide); inhibin beta A chain; Inhibin, beta-1; activin beta-A chain; FSH-releasing protein; erythroid differentiation factor; erythroid differentiation protein; follicle-stimulating hormone-releasing protein; EDF; FRP;
Gene ID : 3624
mRNA Refseq : NM_002192
Protein Refseq : NP_002183
MIM : 147290
UniProt ID : P08476
Chromosome Location : 7p15-p13
Pathway : ALK1 signaling events, organism-specific biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Glycoprotein hormones, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of amino acids and derivatives, organism-specific biosystem; Peptide hormone biosynthesis, organism-specific biosystem;
Function : cytokine activity; follistatin binding; growth factor activity; hormone activity; identical protein binding; protein binding; protein heterodimerization activity; type II activin receptor binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Inquiry

  • Q&As
  • Reviews

Q&As (7)

Ask a question
In which tissues is INHBA predominantly expressed? 06/22/2021

INHBA is often expressed in the ovaries and testes.

What is the role of INHBA in the body? 09/20/2018

INHBA is involved in the regulation of reproductive processes and cell growth.

What is the molecular weight of the INHBA protein? 03/16/2018

The molecular weight is approximately 45 kDa.

Which genes code for the production of INHBA? 11/24/2017

INHBA is encoded by the INHBA gene.

Is INHBA associated with any diseases? 09/08/2017

Mutations in the INHBA gene can be linked to certain reproductive disorders.

What is the full name of the INHBA protein? 09/02/2017

The full name is “Inhibin subunit beta A.”

Does INHBA have any known protein interactions? 08/12/2016

Yes, INHBA can interact with other proteins involved in cell signaling pathways.

Customer Reviews (3)

Write a review
Reviews
12/15/2018

    The value derived from the product's usage made the investment worthwhile, considering the insights acquired.

    08/17/2018

      Its reliability over time reduced the need for frequent reordering, saving both time and resources.

      02/24/2017

        The product’s biocompatibility with various cell types and organisms made it versatile for a wide range of applications.

        Ask a Question for All INHBA Products

        Required fields are marked with *

        My Review for All INHBA Products

        Required fields are marked with *

        0

        Inquiry Basket

        cartIcon
        logo

        FOLLOW US

        Terms and Conditions        Privacy Policy

        Copyright © 2024 Creative BioMart. All Rights Reserved.

        Contact Us

        • /

        Stay Updated on the Latest Bioscience Trends