Recombinant Human MARCKS-like 1, His-tagged

• Cat.No.: MARCKSL1-526H
• Product Overview: MARCKSL1, 1-195aa , Human, His tag, E.coli

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-195 a.a.
• Description: MARCKS-related protein, also known as MARCKSL1, is a member of MARCKS family of PKC substrate. It is widely used in the signal transduction studies as an indicator of PKC activation. Expressed in a variety of tissues with highest levels found in testis and uterus, MARCKSL1 participates in the coordination of membrane-cytoskeletal signaling events, including secretion, migration, phagocytosis and cell adhesion. Additionally, MARCKSL1 functions as a regulator of Integrin activation and is thought to regulate Integrin-dependent signal transduction pathways, especially those involved in macrophage spreading. Recombinant human MARCKSL1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 20% glycerol
• Molecular Mass: 21.9kDa (218aa) confirmed by MALDI-TOF(Molecular weight on SDS-PAGE will appear higher)
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMGSQSSK APRGDVTAEE AAGASPAKAN GQENGHVKSN GDLSPKGEGE SPPVNGTDEA AGATGDAIEP APPSQGAEAK GEVPPKETPK KKKKFSFKKP FKLSGLSFKR NRKEGGGDSS ASSPTEEEQE QGEIGACSDE GTAQEGKAAA TPESQEPQAK GAEASAASEE EAGPQATEPS TPSGPESGPT PASAEQNE
• Purity: >90% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/ml (determined by BCA assay)

Gene Information

• Gene Name: MARCKSL1 MARCKS-like 1 [ Homo sapiens ]
• Official Symbol: MARCKSL1
• Synonyms: MARCKSL1; MARCKS-like 1; MARCKS like protein , MLP; MARCKS-related protein; F52; MacMARCKS; MLP1; mac-MARCKS; MARCKS-like protein 1; macrophage myristoylated alanine-rich C kinase substrate; MLP; MRP; MACMARCKS
• Gene ID: 65108
• mRNA Refseq: NM_023009
• Protein Refseq: NP_075385
• MIM: 602940
• UniProt ID: P49006
• Chromosome Location: 1p35.1
• Pathway: Fc gamma R-mediated phagocytosis, organism-specific biosystem; Fc gamma R-mediated phagocytosis, conserved biosystem; Leishmaniasis, organism-specific biosystem; Leishmaniasis, conserved biosystem
• Function: calmodulin binding