Recombinant Human PDZ domain containing 1, His-tagged

• Cat.No.: PDZK1-560H
• Product Overview: PDZK1, 1-519aa, Human His tag, E.coli

Specification

• Description: PDZK1 is a PDZ domain-containing scaffolding protein. This protein mediates the localization of cell surface proteins and plays a critical role in cholesterol metabolism by regulating the HDL receptor, scavenger receptor class B type 1. Single nucleotide polymorphisms in this gene may be associated with metabolic syndrome, and overexpression of this gene may play a role in drug resistance of multiple myeloma. Recombinant human PDZK1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Source: E.coli
• Species: Human
• Tag: His-tag
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol, 1mM DTT.
• Molecular Mass: 59 kDa (542aa)
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMTSTFNP RECKLSKQEG QNYGFFLRIE KDTEGHLVRV VEKCSPAEKA GLQDGDRVLR INGVFVDKEE HMQVVDLVRK SGNSVTLLVL DGDSYEKAVK TRVDLKELGQ SQKEQGLSDN ILSPVMNGGV QTWTQPRLCY LVKEGGSYGF SLKTVQGKKG VYMTDITPQG VAMRAGVLAD DHLIEVNGEN VEDASHEEVV EKVKKSGSRV MFLLVDKETD KRHVEQKIQF KRETASLKLL PHQPRIVEMK KGSNGYGFYL RAGSEQKGQI IKDIDSGSPA EEAGLKNNDL VVAVNGESVE TLDHDSVVEM IRKGGDQTSL LVVDKETDNM YRLAHFSPFL YYQSQELPNG SVKEAPAPTP TSLEVSSPPD TTEEVDHKPK LCRLAKGENG YGFHLNAIRG LPGSFIKEVQ KGGPADLAGL EDEDVIIEVN GVNVLDEPYE KVVDRIQSSG KNVTLLVCGK KAYDYFQAKK IPIVSSLADP LDTPPDSKEG IVVESNHDSH MAKERAHSTA SHSSSNSEDT EM
• Purity: >90% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: PDZK1 PDZ domain containing 1 [ Homo sapiens ]
• Official Symbol: PDZK1
• Synonyms: PDZK1; PDZ domain containing 1; Na(+)/H(+) exchange regulatory cofactor NHE-RF3; NHERF3; PDZD1; naPi-Cap1; PDZ-containing kidney protein 1; CFTR-associated protein of 70 kDa; sodium-hydrogen exchanger regulatory factor 3; na/Pi cotransporter C-terminal-associated protein 1; CAP70; CLAMP; NHERF-3
• Gene ID: 5174
• mRNA Refseq: NM_001201325
• Protein Refseq: NP_001188254
• MIM: 603831
• UniProt ID: Q5T2W1
• Chromosome Location: 1q21
• Function: PDZ domain binding; protein binding; scavenger receptor binding; transporter activity