Recombinant Human cyclin-dependent kinase 1, His-tagged CDK1-654H

Recombinant Human cyclin-dependent kinase 1, His-tagged

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Recombinant Human cyclin-dependent kinase 1, His-tagged

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Cat.No. : CDK1-654H
Product Overview : CDK1, 1-297aa Human, His tag, Insect cell
Description : Cyclin-dependent kinase 1, also known as CDK1, plays a key role in the control of the eukaryotic cell cycle by modulating the centrosome cycle as well as mitotic onset; promotes G2-M transition, and regulates G1 progress and G1-S transition via association with multiple interphase cyclins. This protein is required in higher cells for entry into S-phase and mitosis. Recombinant human CDK1 protein, fused to His-tag at C-terminus, was expressed in insect cells using baculovirus expression system and purified by using conventional chromatography techniques.
Source : Insect cell
Species : Human
Tag : His-tag
Form : Liquid. In Phosphate buffered saline (pH7.4), 30% glycerol, 2mM DTT, 0.1mM PMSF
Molecular Mass : 35.1 kDa (305aa)
AA Sequence : MEDYTKIEKI GEGTYGVVYK GRHKTTGQVV AMKKIRLESE EEGVPSTAIR EISLLKELRH PNIVSLQDVL MQDSRLYLIF EFLSMDLKKY LDSIPPGQYM DSSLVKSYLY QILQGIVFCH SRRVLHRDLK PQNLLIDDKG TIKLADFGLA RAFGIPIRVY THEVVTLWYR SPEVLLGSAR YSTPVDIWSI GTIFAELATK KPLFHGDSEI DQLFRIFRAL GTPNNEVWPE VESLQDYKNT FPKWKPGSLA SHVKNLDENG LDLLSKMLIY DPAKRISGKM ALNHPYFNDL DNQIKKMLEH HHHHH
Purity : >95% by SDS - PAGE
Storage : Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Concentration : 0.5 mg/ml (determined by Bradford assay)
Gene Name : CDK1 cyclin-dependent kinase 1 [ Homo sapiens ]
Official Symbol : CDK1
Synonyms : CDK1; cyclin-dependent kinase 1; CDC2, cell division cycle 2, G1 to S and G2 to M; CDC28A; p34 protein kinase; cell cycle controller CDC2; cell division protein kinase 1; cell division control protein 2 homolog; cell division cycle 2, G1 to S and G2 to M; CDC2; P34CDC2; MGC111195; DKFZp686L20222;
Gene ID : 983
mRNA Refseq : NM_001170406
Protein Refseq : NP_001163877
MIM : 116940
UniProt ID : P06493
Chromosome Location : 10q21.2
Pathway : APC/C-mediated degradation of cell cycle proteins, organism-specific biosystem; APC/C:Cdc20 mediated degradation of Cyclin B, organism-specific biosystem; APC/C:Cdc20 mediated degradation of mitotic proteins, organism-specific biosystem; ARMS-mediated activation, organism-specific biosystem; Activated TLR4 signalling, organism-specific biosystem; Activation of APC/C and APC/C:Cdc20 mediated degradation of mitotic proteins, organism-specific biosystem; Androgen Receptor Signaling Pathway, organism-specific biosystem;
Function : ATP binding; Hsp70 protein binding; RNA polymerase II carboxy-terminal domain kinase activity; cyclin binding; cyclin-dependent protein kinase activity; cyclin-dependent protein kinase activity; histone kinase activity; nucleotide binding; protein binding

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