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Recombinant Human mitochondrial ribosomal protein L2, His-tagged

Cat.No.: MRPL2-681H
Product Overview: MRPL2, 84-202aa Human, His tag, E.coli
Description: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. MRPL2 is a 39S subunit protein that belongs to the EcoL2 ribosomal protein family. Recombinant human MRPL2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Source: E.coli
Species: Human
Tag: His-tag
Form: Liquid. In 20mM Phosphate buffer (pH 8.0) containing 1mM EDTA, 50% glycerol, 2mM DTT.
Molecular Mass: 15.5 kDa (142aa) confirmed by MALDI-TOF.
AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSGRDHTGR IRVHGIGGGH KQRYRMIDFL RFRPEETKSG PFEEKVIQVR YDPCRSADIA LVAGGSRKRW IIATENMQAG DTILNSNHIG RMAVAAREGD AHPLGALPVG TLINNVESEP GR
Purity: >90% by SDS - PAGE
Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Concentration: 0.25 mg/ml (determined by Bradford assay)
Gene Name: MRPL2 mitochondrial ribosomal protein L2 [ Homo sapiens ]
Official Symbol: MRPL2
Synonyms: MRPL2; mitochondrial ribosomal protein L2; RPML14; MRP-L14;
Gene ID: 65007

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