Recombinant Human Smad nuclear interacting protein 1, His-tagged

• Cat.No.: SNIP1-692H
• Product Overview: SNIP1, 258-396aa, Human, His tag, E.coli

Specification

• Description: SNIP1 is smad nuclear interacting protein contains a forkhead-associated (FHA) domain and acts as a nuclear inhibitor of CBP/p300. This protein is an inhibitor of the TGF-beta signal transduction pathway and to be important in suppressing transcriptional activation dependent on the co-activators CBP and p300. Inhibition of NF-kappa B activity is a function of the N-terminal domain of SNIP1 and involves competition of SNIP1 and the NF-kappa B subunit, RelA/p65, for binding to p300, similar to the mechanism of inhibition of Smad signaling by SNIP1. Recombinant human SNIP1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Source: E.coli
• Species: Human
• Tag: His-tag
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 20% glycerol, 100mM NaCl
• Molecular Mass: 18.8kDa (160aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MRWRLYPFKN DEVLPVMYIH RQSAYLLGRH RRIADIPIDH PSCSKQHAVF QYRLVEYTRA DGTVGRRVKP YIIDLGSGNG TFLNNKRIEP QRYYELKEKD VLKFGFSSRE YVLLHESSDT SEIDRKDDED EEEEEEVSDS
• Purity: >90% by SDS - PAGE
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 1mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: SNIP1 Smad nuclear interacting protein 1 [ Homo sapiens ]
• Official Symbol: SNIP1
• Synonyms: SNIP1; Smad nuclear interacting protein 1; smad nuclear-interacting protein 1; FHA domain-containing protein SNIP1; PMRED; FLJ12553
• Gene ID: 79753
• mRNA Refseq: NM_024700
• Protein Refseq: NP_078976
• MIM: 608241
• UniProt ID: Q8TAD8
• Chromosome Location: 1p34.3
• Pathway: Regulation of nuclear SMAD2/3 signaling, organism-specific biosystem; TGF-beta Receptor Signaling Pathway, organism-specific biosystem
• Function: protein binding