Recombinant Rhesus macaque EPCAM protein, His-tagged

• Cat.No.: EPCAM-202C
• Product Overview: Recombinant Rhesus macaque EPCAM (Gln24-Lys265) protein was fused to His-tag at C-terminus and expressed in human 293 cells (HEK293).

Specification

• Description: EpCAM is also known as CO171A, EGP, EGP40,GA7332, KSA, M4S, MIC18, MK1, TROP1, hEGP2, and is a pan-epithelial differentiation antigen that is expressed on almost all carcinomas as 17-1A(mAb) antigen. Its constitutional function is being elucidated. It is intricately linked with the Cadherin-Catenin pathway and hence the fundamental WNT pathway responsible for intracellular signaling and polarity. The epithelial cell adhesion molecule (Ep-CAM) is known to express in most epithelial malignancies and was reported as a tumor marker or a candidate of molecular targeting therapy.
  Ep-CAM cross signaling with N-cadherin involves Pi3K, resulting in the abrogation of the cadherin adhesion complexes in epithelial cells was reported. And Epithelial cell adhesion molecule (Ep-CAM) recently received increased attention as a prognostic factor in breast cancer.
• Source: HEK293
• Species: Rhesus macaque
• Tag: His
• Predicted N Terminal: Gln24
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
• Molecular Mass: The protein has a calculated MW of 28.2 kDa. The protein migrates as 28-40 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation.
• Protein length: Gln24-Lys265
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: EPCAM
• Official Symbol: EPCAM
• Synonyms: Ep-CAM; TACSTD1
• Gene ID: 677680
• mRNA Refseq: NM_001040029.1
• Protein Refseq: NP_001035118.1
• UniProt ID: Q1WER1