| Source : |
HEK293 |
| Species : |
Cynomolgus |
| Tag : |
N-hFc |
| Protein length : |
Gly136-Asp317 |
| Form : |
Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
| Bio-activity : |
1. Immobilized Cynomolgus S4-Fc3L3-TNFSF11 at 10 μg/ml (100 μl/well) can bind biotinylated human TNFRSF11B-His, The EC50 of biotinylated human TNFRSF11B-His is 7.94-18.52 ng/ml. 2. The bioactivity of RANKL was determined by measuring the ability of RANKL to induce TRAP activity in RAW264.7 cells. The ED50 for this effect is typically 7-35 ng/mL. |
| Molecular Mass : |
The recombinant Cynomolgus TNFSF11 comprises 442 amino acids and has a calculated molecular mass of 48.9 KDa. The apparent molecular mass of the protein is approximately 55 KDa in SDS-PAGE. |
| Endotoxin : |
< 1.0 EU per μg of the protein as determined by the LAL method |
| Purity : |
> 85 % as determined by SDS-PAGE |
| Storage : |
Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
