Active Recombinant Human CTF1 protein(Ser2-Ala201)

• Cat.No.: CTF1-238H
• Product Overview: Recombinant Human CTF1 (NP_001321.1) (Ser2-Ala201) was expressed in HEK293 with two additional amino acids (Gly & Pro) at the N-terminus.

Specification

• Source: HEK293
• Species: Human
• Tag: N/A
• Protein length: Ser2-Ala201
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 2-10 ng/ml.
• Molecular Mass: The recombinant human CTF1 comprises 202 amino acids and has a predicted molecular mass of 21.2 kDa. The apparent molecular mass of the protein is approximately 26.6 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 85 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: CTF1 cardiotrophin 1 [ Homo sapiens ]
• Official Symbol: CTF1
• Synonyms: CTF1; cardiotrophin 1; cardiotrophin-1; CT 1; CT1; cardiophin 1; CT-1
• Gene ID: 1489
• mRNA Refseq: NM_001142544
• Protein Refseq: NP_001136016
• MIM: 600435
• UniProt ID: Q16619