Active Recombinant Mouse Sparcl1 protein, His-tagged

• Cat.No.: Sparcl1-536M
• Product Overview: Recombinant Mouse Sparcl1 protein(EDL20231.1)(Met1-Phe650), fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Mouse
• Tag: His
• Form: Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: Measured by its ability to inhibit the cell growth of Mv-1-Lu mink lung epithelial cells. The ED50 for this effect is typically 1-4 μg/mL.
• Molecular Mass: The recombinant mouse SPARCL1 comprises 645 amino acids and has a predicted molecular mass of 72.2 kDa. The apparent molecular mass of the protein is approximately 118 kDa in SDS-PAGE under reducing conditions.
• Proteinlength: Met1-Phe650
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 85 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Sparcl1 SPARC-like 1 [ Mus musculus ]
• Official Symbol: Sparcl1
• Synonyms: SPARCL1; SPARC-like 1; SPARC-like protein 1; matrix glycoprotein Sc1; SPARC-like 1 (mast9, hevin); extracellular matrix protein 2; Sc1; Ecm2; hevin; mast9
• Gene ID: 13602
• mRNA Refseq: NM_010097
• Protein Refseq: NP_034227