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Active Recombinant Rat Epha3 protein(Met1-His541), His-tagged

Cat.No. : Epha3-552R
Product Overview : Recombinant Rat EPHA3 (EDL75897.1) (Met1-His541) was expressed in HEK293 with a polyhistidine tag at the C-terminus.
  • Specification
  • Gene Information
  • Related Products
Source : HEK293
Species : Rat
Tag : C-His
Protein length : Met1-His541
Form : Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Bio-activity : Measured by its binding ability in a functional ELISA. Immobilized rat EPHA3-His at 10 μg/ml (100 μl /well) can bind rat EFNA5-Fc, The EC50 of rat EFNA5-Fc is 9-20ng/ml.
Molecular Mass : The recombinant rat EPHA3 comprises 532 amino acids and predicts a molecular mass of 61.2 kDa. The apparent molecular mass of the recombinant protein is approximately 70.5 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method
Purity : > 90 % as determined by SDS-PAGE
Storage : Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
Gene Name : Epha3 Eph receptor A3 [ Rattus norvegicus ]
Official Symbol : Epha3
Synonyms : EPHA3; Eph receptor A3; ephrin type-A receptor 3; EK4; rEK4; EPH-like kinase 4; tyrosine-protein kinase TYRO4; tyrosine-protein kinase receptor REK4;
Gene ID : 29210
mRNA Refseq : NM_031564
Protein Refseq : NP_113752

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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