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Recombinant Rat ALT, His-tagged

Cat.No. : ALT-3248R
Product Overview : Alanine Aminotransferase (ALT)
  • Specification
  • Gene Information
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Description : human homolog is a cytosolic alanine aminotransferase .
Source : E. Coli
Species : Rat
Tag : His
Form : Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Molecular Mass : 56.6kDa
Protein length : 496
Endotoxin : <1.0eu per 1ug (determined by the lal</1.0eu
Purity : >95%
Applications : SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
Notes : Small volumes of ALT recombinant protein may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice.
Storage : Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
Reconstitution : Reconstitute in sterile PBS, pH7.2-pH7.4.
Warning : This product is for research use only. Not for use in diagnostic or therapeutic procedures.
Gene Name : Gpt glutamic-pyruvate transaminase (alanine aminotransferase) [ Rattus norvegicus ]
Official Symbol : Gpt
Synonyms : Gpt1; alanine aminotransferase 1; ALT1; GPT 1; glutamic pyruvate transaminase; glutamic--alanine transaminase 1; glutamic--pyruvic transaminase 1; glutamate pyruvate transaminase 1; glutamic pyruvic transaminase 1, soluble; glutamic pyruvic transaminase (alanine aminotrasferase)
Gene ID : 81670
mRNA Refseq : NM_031039
Protein Refseq : NP_112301
UniProt ID : P25409
Chromosome Location : 7q34
Pathway : 2-Oxocarboxylic acid metabolism, organism-specific biosystem; Amino acid synthesis and interconversion (transamination), organism-specific biosystem
Function : L-alanine:2-oxoglutarate aminotransferase activity; pyridoxal phosphate binding

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (5)

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What role does the GPT gene, including GPT1 and GPT2 subtypes, play in glucose and amino acid metabolism, and how is it implicated in cancer-related tumorigenesis? 05/01/2022

The GPT gene, encompassing GPT1 and GPT2, is pivotal in glucose and amino acid metabolism. GPT2 is notably linked to tumorigenesis in various cancers, while the involvement of GPT1 in cancer remains uncertain and requires further exploration.

What role does the GPT gene, encoding cytosolic alanine aminotransaminase 1 (ALT1), play in cellular metabolism and liver function? 04/13/2021

The GPT gene encodes ALT1, a key enzyme in cellular metabolism. ALT1 facilitates the reversible transamination between alanine and 2-oxoglutarate, crucial for glucose and amino acid metabolism. Elevated serum ALT1 levels are commonly used as a biomarker for liver injury caused by drug toxicity, infection, alcohol, and steatosis.

How does the combined SMS and GPT signature affect LSCC patient prognosis, and what factors explain the discrepancy between SMS transcriptional and protein levels? 08/04/2020

SMS protein alone doesn't impact LSCC patient prognosis, but when combined with GPT, it shows significance. Inconsistency between SMS transcriptional and protein levels may stem from various factors, including the involvement of regulatory elements and different post-transcriptional and post-translational modifications.

How does GPT gene expression, assessed in LSCC tissues and cell lines, correlate with SMS and impact overall survival rates in LSCC patients? 01/06/2019

Analysis of LSCC tissues and cell lines revealed an inverse relationship between GPT and SMS expressions, confirmed through qRT-PCR and TCGA data. GPT protein, primarily located in the epithelium, was down-regulated in LSCC tissues.

How does GPT gene expression in tissues like skeletal muscle, kidney, and liver reflect cancer-related metabolic changes? 05/12/2018

GPT gene expression serves as a marker for cancer-induced metabolic reprogramming in tissues, indicating shifts in energy metabolism and biosynthetic processes.

Customer Reviews (3)

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Reviews
11/13/2021

    Fantastic customer support. They genuinely care about the success of our research.

    10/11/2020

      Top-notch protein quality! It's our go-to for reliable and reproducible results.

      04/06/2018

        Very happy with this lab product!

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