| Description : |
Interleukin-19 (IL-19) belongs to the IL-10 family that includes IL-10, IL-20, IL-22, IL-24, and IL-26. As a monomer made of seven amphipathic helices, IL-19 has a helical bundle and shares the same cell surface receptor (IL-20R) with IL-20 and IL-24. It may play some important roles in inflammatory responses because it up-regulates IL-6 and TNF-alpha and induces apoptosis. |
| Source : |
E.coli |
| Species : |
Mouse |
| Form : |
Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH 7.4, with 3 % Trehalose. |
| Bio-activity : |
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human IL-20Rα and human IL-20Rβ co-transfected murine BaF3 pro-B cells is less than 0.8 ng/ml, corresponding to a specific activity of > 1.25 × 10⁶ IU/mg. |
| Molecular Mass : |
Approximately 17.6 kDa, a single non-glycosylated polypeptide chain containing 152 amino acids. |
| Protein length : |
152 |
| AA Sequence : |
LRRCLISVDMRLIEKSFHEIKRAMQ TKDTFKNVTILSLENLRSIKPGDVC CMTNNLLTFYRDRVFQDHQERSLEV LRRISSIANSFLCVQKSLERCQVHR QCNCSQEATNATRIIHDNYNQLEVS SAALKSLGELNILLAWIDRNHLETP AA |
| Endotoxin : |
Less than 0.1 EU/µg of rMuIL-19 as determined by LAL method. |
| Purity : |
>97% by SDS-PAGE and HPLC analysis. |
| Storage : |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution. |
| Reconstitution : |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions. |
