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Recombinant Mouse Interleukin 20

Cat. No.: Il20-74M
Product Overview: Recombinant Mouse Interleukin-20 produced inE.Coliis a single, non-glycosylated polypeptide chain containing 152 amino acids and having a total molecular mass of 17,584 Dalton. The IL-20 is purified by proprietary chromatographic techniques.
Description: IL20 is a cytokine structurally related to interleukin 10 (IL10). IL20 cytokine has been shown to transduce its signal through signal transducer and activator of transcription 3 (STAT3) in keratinocytes. A specific receptor for this cytokine is found to be expressed in skin and upregulated dramatically in psoriatic skin, suggesting a role for this protein in epidermal function and psoriasis.
Source: Escherichia Coli.
Amino Acid Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Leu-Lys-Thr-Leu.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Formulation: Lyophilized from a concentrated (1 mg/ml) solution containing no additives.
Solubility: It is recommended to reconstitute the lyophilized Interleukin-20 in sterile 18 MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Specific Activity: The biological activity was determined by measuring the dose dependent activation of STAT3 in human epidermal keratinocytes (HEK2a cells). Significant STAT3 activation is observed with >100 ng/mL of Interleukin-20 recombinant mouse.
Protein Content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.552 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of IL-20 as a Reference Standard.
Stability: Lyophilized Interleukin-20 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL20 should be stored at 4°Cbetween 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Gene Name: Il20 interleukin 20 [ Mus musculus ]
Synonyms: Il20; interleukin 20; Zcyto10; MGC130536; four helix bundle cytokine 10
Gene ID: 58181
mRNA Refseq: NM_021380
Protein Refseq: NP_067355
UniProt ID: Q9JKV9
Chromosome Location: 1 E4
Pathway: Cytokine-cytokine receptor interaction; Jak-STAT signaling pathway
Function: cytokine activity; interleukin-20 receptor binding; receptor binding

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