Description : |
The protein encoded by this gene belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. The glycoprotein subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes a beta 3 subunit. This gene encodes a beta 3 subunit. A pseudogene exists for this gene, and it is located on chromosome 2. |
Source : |
E. Coli |
Species : |
Human |
Tag : |
His,T7 |
Form : |
Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl. |
Molecular Mass : |
27.7kDa |
Protein length : |
279 |
Endotoxin : |
<1.0eu per 1ug (determined by the lal</1.0eu |
Purity : |
>95% |
Applications : |
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP) |
Notes : |
Small volumes of ATP1b3 recombinant protein may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice. |
Storage : |
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition. |
Reconstitution : |
Reconstitute in sterile PBS, pH7.2-pH7.4. |
Warning : |
This product is for research use only. Not for use in diagnostic or therapeutic procedures. |