Recombinant Human DOT1L, GST-tagged

• Cat.No.: DOT1L-658H
• Product Overview: Recombinant human DOT1 protein, catalytic domain (DOT1L, KMT4, accession Q8TEK3, amino acids 1-472), containing an N-terminal GST tag, expressed in E. coli

Specification

• Description: The protein encoded by this gene is a histone methyltransferase that methylates lysine-79 of histone H3. It is inactive against free core histones, but shows significant histone methyltransferase activity against nucleosomes.
• Source: E. coli
• Species: Human
• Tag: GST
• Form: Recombinant GST-DOT1L (1 µg/µl) 100 mM Tris pH 8.0, 5 mM glutathione and 25% glycerol.
• Molecular Mass: 85 kDa
• Purity: 70% as measured by gel densitometry.
• Applications: Recombinant DOT1L Catalytic Domain, human is useful for histone H3 methylation experiments, enzyme kinetics and inhibitor screening. Use of 3-5 µg DOT1L per reaction with HeLa or recombinant nucleosomes as a substrate is recommended.
• Storage: Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.

Gene Information

• Gene Name: DOT1L DOT1-like histone H3K79 methyltransferase [ Homo sapiens (human) ]
• Official Symbol: DOT1L
• Synonyms: DOT1L; DOT1; KMT4; DOT1-like histone H3K79 methyltransferase; histone-lysine N-methyltransferase, H3 lysine-79 specific; H3-K79-HMTase; DOT1-like protein; lysine N-methyltransferase 4; histone methyltransferase DOT1L; histone H3-K79 methyltransferase; DOT1-like, histone H3 methyltransferase; NP_115871.1; EC 2.1.1.43
• Gene ID: 84444
• mRNA Refseq: NM_032482
• Protein Refseq: NP_115871
• MIM: 607375
• UniProt ID: Q8TEK3
• Chromosome Location: 19p13.3
• Pathway: Lysine degradation; Transcriptional misregulation in cancer
• Function: DNA binding; histone-lysine N-methyltransferase activity; protein binding