Recombinant Porcine Interleukin-15
Cat.No. : | IL15-80P |
Product Overview : | Recombinant Porcine Interleukin-15 produced inE.Coliis a single, non-glycosylated polypeptide chain containing 114 amino acids and having a molecular mass of 13,118 Dalton. The IL-15 is purified by proprietary chromatographic techniques. |
- Specification
- Gene Information
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Cat. No. : | IL15-80P |
Description : | Interleukin 15 (IL-15) is a cytokine with structural similarity to IL-2. Like IL-2, IL-15 binds to and signals through the IL-2/IL-15 beta chain (CD122) and the common gamma chain (gamma-C, CD132). IL-15 is secreted by mononuclear phagocytes (and some other cells) following infection by virus(es). |
Source : | Escherichia Coli. |
Amino Acid Sequence : | The sequence of the first five N-terminal amino acids was determined and was found to be Thr-Trp-Gln-His-Val. |
Physical Appearance : | Sterile Filtered White lyophilized (freeze-dried) powder. |
Purity : | Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
Formulation : | The protein was lyophilized from a concentrated (1 mg/ml) solution containing no additives. |
Solubility : | It is recommended to reconstitute the lyophilized Interleukin-15 in sterile 18 MΩ-cm H2O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions. |
Specific Activity : | The ED50 as determined by the dose-dependant stimulation of the proliferation of mouse CTLL-2 was found to be < 0.2 ng/ml. |
Protein Content : | Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.638 as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is calculated by the PC GENEcomputer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of IL-15 as a Reference Standard. |
Stability : | Lyophilized Interleukin-15 although stable at room temperature for 3 weeks, should be stored desiccated below -18℃. Upon reconstitution IL15 should be stored at 4℃ between 2-7 days and for future use below -18℃. For long term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
Pathways : | Cytokine-cytokine receptor interaction; Intestinal immune network for IgA production; Jak-STAT signaling pathway |
Gene Name : | IL15 interleukin 15 [ Sus scrofa ] |
Synonyms : | IL15; interleukin 15; IL-15; interleukin-15 |
Gene ID : | 397683 |
mRNA Refseq : | NM_214390 |
Protein Refseq : | NP_999555 |
UniProt ID : | Q95253 |
Products Types
◆ Lysates | ||
IL15-5248HCL | Recombinant Human IL15 293 Cell Lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (10)
Ask a questionTechniques discern IL15 and IL2 receptor binding kinetics.
Methods explore IL15's role in autoimmune disease mechanisms.
IL15 knockout models provide insights into its diverse functions.
IL15's influence on memory T cell differentiation investigated.
Immunofluorescence microscopy visualizes IL15 subcellular localization.
Approaches validate IL15's involvement in inflammatory responses.
IL15's role in NK cell cytotoxicity analyzed using assays.
ELISA measures IL15 secretion accurately.
qPCR quantifies IL15's impact on regulatory T cells.
Flow cytometry quantifies IL15-driven T-cell proliferation.
Customer Reviews (3)
Write a reviewMapping protein- interactions in DNA transcription for gene regulation.
Suitable for cytotoxicity assays.
Effective in NK cell activation.
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