Active Recombinant Human IL10RA, HIgG1 Fc-tagged

• Cat.No.: IL10RA-212H
• Product Overview: The extracellular domain of human IL-10RA (AAH28082.1) (His22-Asn235) is fused to the N-terminus of the Fc region of a human IgG1 was expressed in CHO cell.

Specification

• Description: The protein encoded by this gene is a receptor for interleukin 10. This protein is structurally related to interferon receptors. It has been shown to mediate the immunosuppressive signal of interleukin 10, and thus inhibits the synthesis of proinflammatory cytokines. This receptor is reported to promote survival of progenitor myeloid cells through the insulin receptor substrate-2/PI 3-kinase/AKT pathway. Activation of this receptor leads to tyrosine phosphorylation of JAK1 and TYK2 kinases. Two transcript variants, one protein-coding and the other not protein-coding, have been found for this gene.
• Source: CHO cell
• Species: Human
• Tag: HIgG1 Fc-tagged
• Form: Lyophilized from 0.2μm-filtered solution in PBS.
• Bio-activity: Measured by its ability to inhibit IL-10-dependent proliferation of MC/9 2 mouse mast cells.
• Molecular Mass: 51KDa (monomer)
• AA Sequence: His22-Asn235
• Endotoxin: <0.06 eu/μg as determined by lal test.>
• Purity: >98%, by SDS-PAGE under reducing conditions.
• Stability: Stable for at least 1 year after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.
• Reconstitution: Reconstitute at 100μg/ml in sterile PBS.
• Warning: Avoid freeze/thaw cycles.
• Publication:
  Investigation the Possibility of Using Peptides with a Helical Repeating Pattern of Hydro-Phobic and Hydrophilic Residues to Inhibit IL-10 (2016)
  Developing a novel therapeutic vaccine combining IL-10 inhibitor for late stage cancers with targeted Clostridial spore oncolysis of the tumour microenvironment (2017)

Gene Information

• Gene Name: IL10RA interleukin 10 receptor, alpha [ Homo sapiens ]
• Official Symbol: IL10RA
• Synonyms: IL10RA; interleukin 10 receptor, alpha; IL10R; interleukin-10 receptor subunit alpha; CD210; CD210a; CDW210A; HIL 10R; IL-10RA; IL-10R subunit 1; IL-10R subunit alpha; IL-10 receptor subunit alpha; interleukin-10 receptor subunit 1; interleukin-10 receptor alpha chain; HIL-10R; IL-10R1
• Gene ID: 3587
• mRNA Refseq: NM_001558
• Protein Refseq: NP_001549
• MIM: 146933
• UniProt ID: Q13651
• Chromosome Location: 11q23
• Pathway: Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Jak-STAT signaling pathway, organism-specific biosystem; Jak-STAT signaling pathway, conserved biosystem; Toxoplasmosis, organism-specific biosystem; Toxoplasmosis, conserved biosystem; Tuberculosis, organism-specific biosystem
• Function: interleukin-10 receptor activity; protein binding; receptor activity; signal transducer activity