Ni-NTA Agarose
| Cat.No. : | Ni-NTA-001A |
| Product Overview : | Ni-NTA is designed for high quality purification of 6xhis-tagged recombinant proteins from bacteria, insect and mammalian cells. Ni-NTA agarose beads are widely used for protein purification due to its high affinity and selectivity for recombinant fusion proteins that are tagged with six tandem histidine residues. |
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| Cat.no : | Ni-NTA-001A |
| Characteristics : | NTA binds Ni2+ ions by four coordination sites. The Ni-NTA Agarose exhibits high affinity and selectivity for 6xhis-tagged recombinant fusion proteins, which can be purified under native, denaturing, or hybrid conditions using the Ni-NTA Agarose. Proteins bound to the resin are eluted with low pH buffer or by competition with imidazole or histidine. |
| Form : | It is provided as a 50% slurry in 30% ethanol. |
| Size : | 10 mL, 25 mL, 100 mL |
| Matrix : | 4% cross-linked agarose |
| Average particle size : | 45-165 μm |
| Ligand : | Nitrilotriacetic acid (NTA) |
| Dynamic binding capacity : | 5-10 mg of protein per ml of resin |
| Recommended flow rate : | 30 ml/h |
| Recommended column height : | 5‐20cm |
| Chemical stability : | Stable to all solutions commonly used in gel filtration including reducing agents |
| Physical stability : | Ni-NTA resin is guaranteed stable for 6 months when properly stored. |
| pH working range : | 3-13 |
| pH CIP range : | 2-14 |
| Temperature Stability : | 4‐40 °C |
| Warning : | Avoid using protease inhibitors or other additives that contain chelators, such as EDTA, or strong reducing agents, such as DTT, which will disrupt the function of nikel resin. |
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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