"Ni-NTA" Related Products

      Ni-NTA Agarose

      Cat.no : Ni-NTA-001A
      Product Overview : Ni-NTA is designed for high quality purification of 6xhis-tagged recombinant proteins from bacteria, insect and mammalian cells. Ni-NTA agarose beads are widely used for protein purification due to its high affinity and selectivity for recombinant fusion proteins that are tagged with six tandem histidine residues.
      Characteristics : NTA binds Ni2+ ions by four coordination sites. The Ni-NTA Agarose exhibits high affinity and selectivity for 6xhis-tagged recombinant fusion proteins, which can be purified under native, denaturing, or hybrid conditions using the Ni-NTA Agarose. Proteins bound to the resin are eluted with low pH buffer or by competition with imidazole or histidine.
      Form : It is provided as a 50% slurry in 30% ethanol.
      Size : 10 mL, 25 mL, 100 mL
      Matrix : 4% cross-linked agarose
      Average particle size : 45-165 μm
      Ligand : Nitrilotriacetic acid (NTA)
      Dynamic binding capacity : 5-10 mg of protein per ml of resin
      Recommended flow rate : 30 ml/h
      Recommended column height : 5‐20cm
      Chemical stability : Stable to all solutions commonly used in gel filtration including reducing agents
      Physical stability : Ni-NTA resin is guaranteed stable for 6 months when properly stored.
      pH working range : 3-13
      pH CIP range : 2-14
      Temperature Stability : 4‐40 °C
      Warning : Avoid using protease inhibitors or other additives that contain chelators, such as EDTA, or strong reducing agents, such as DTT, which will disrupt the function of nikel resin.

      Online Inquiry

      Note: There will be extra charge for optional service!

      Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information

      Optional requirements on this protein    +Expand

      Price Inquiry

      Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.